The Rho GTPase activators CNF1 and DNT bacterial toxins have mucosal adjuvant properties. - Archive ouverte HAL Access content directly
Journal Articles Vaccine Year : 2005

The Rho GTPase activators CNF1 and DNT bacterial toxins have mucosal adjuvant properties.

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Abstract

Cytotoxic necrotizing factor 1 (CNF1) from uropathogenic Escherichia coli belongs to a family of factors activating Rho GTPases. We report the in vivo effects of CNF1 in mice co-fed toxin and the soluble protein antigen ovalbumin (OVA). Similar to cholera toxin, CNF1 elicits adjuvanticity anti-OVA responses, both systemic and mucosal. In contrast, the catalytic inactive mutant CNF1-C866S demonstrated no effects. Using dermonecrotic toxin (DNT), a closely related Rho activating toxin from Bordetella, we discovered that the adjuvant property is within the DNT catalytic domain. Manipulation of Rho proteins thus provides a possible new approach for the development of effective mucosal immunoadjuvants.

Dates and versions

inserm-00000099 , version 1 (03-04-2006)

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Patrick Munro, Gilles Flatau, Fabienne Anjuere, Véronique Hofman, Cecil Czerkinsky, et al.. The Rho GTPase activators CNF1 and DNT bacterial toxins have mucosal adjuvant properties.. Vaccine, 2005, 23, pp.2551-6. ⟨10.1016/j.vaccine.2004.11.042⟩. ⟨inserm-00000099⟩
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