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1: in vitro models of human villous and extravillous cytotrophoblasts ,
PAPP-A expression in human cultured VCT and EVCT ,
Values were normalized to PPIA transcript levels. Values represent the mean ± SEM of 3 to 5 independent cultures obtained from different placentas. Supernatants from 24, 48 and 72 h-cultured VCT and EVCT were collected and PAPP-A measured as described in Materials and Methods. Protein secretions were expressed as UI/L and normalized to DNA content for comparison between VCT and EVCT. Values represent the mean ± SEM of three independent cultures obtained from three different placentas. Mann and Whitney U test was used for statistical analysis ,