Skip to Main content Skip to Navigation
Journal articles

Modulation of PAPP-A expression by PPARgamma in human first trimester trophoblast.

Abstract : Pregnancy-associated plasma protein-A (PAPP-A) is a metzincin metalloproteinase that cleaves the insulin-like growth factor (IGF)-dependent binding protein-4 and increases in maternal serum during pregnancy. In human placenta PAPP-A is expressed both in villous cytotrophoblasts (VCT) that cover the chorionic villi and in extravillous cytotrophoblasts (EVCT) of the anchoring villi. Due to the key role of PPARgamma in human trophoblast differentiation such as syncytiotrophoblast formation and EVCT invasion, we studied the effect of PPARgamma activation on PAPP-A expression using our in vitro model of EVCT and VCT primary cultures isolated from the same first trimester chorionic villi. First, we demonstrated that invasive EVCT expressed and secreted 10 times more PAPP-A than VCT did. Then, we showed that activation of PPARgamma inhibited PAPP-A gene expression and secretion in EVCT, whereas it had no effect in VCT. Since we have previously shown that PPARgamma agonist inhibits EVCT invasion in vitro, we suggest that PPARgamma-mediated inhibition of PAPP-A might decrease the amount of bioactive IGFII, a factor known to promote trophoblast invasion.
Document type :
Journal articles
Complete list of metadatas

Cited literature [48 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00000053
Contributor : Danièle Evain-Brion <>
Submitted on : Friday, June 2, 2006 - 3:01:07 PM
Last modification on : Tuesday, September 22, 2020 - 3:38:00 AM
Long-term archiving on: : Saturday, April 3, 2010 - 8:47:32 PM

Identifiers

Collections

Citation

Karen Handschuh, Jean Guibourdenche, Mickaël Guesnon, Ingrid Laurendeau, Danièle Evain-Brion, et al.. Modulation of PAPP-A expression by PPARgamma in human first trimester trophoblast.. Placenta, Elsevier, 2006, 27 Suppl A, pp.S127-34. ⟨10.1016/j.placenta.2005.10.012⟩. ⟨inserm-00000053⟩

Share

Metrics

Record views

794

Files downloads

1306