Skip to Main content Skip to Navigation
Journal articles

Human Chorionic Gonadotropin Expression in Human Trophoblasts from Early Placenta: Comparative Study Between Villous and Extravillous Trophoblastic Cells.: hCG in invasive extravillous cytotrophoblast

Abstract : Human trophoblast differentiates into two pathways: extravillous cytotrophoblasts (EVCT) that invade the uterus wall and villous cytotrophoblasts (VCT) that fuse to form the syncytiotrophoblast (ST) involved in placental exchanges and endocrine function. It is established that hCG is produced and secreted by the ST into the maternal compartment where it plays a key endocrine role and stimulates ST formation in an autocrine manner. Herein, we investigated hCG expression in early placentas by immunohistochemistry using different antibodies. We then compared hCG secretion by primary cultures of VCT and EVCT isolated from the same first trimester human chorionic villi. In situ hCG was immunodetected in EVCT all along their invasive differentiating pathway except in cells near the stromal core of the proximal column. hCG expression was confirmed in vitro by immunocytochemistry and hCG secretion quantified in cell supernatants. Interestingly, whereas hCG secretion increased during VCT differentiation into ST (from 60 to 350UI/L/mug DNA), EVCT secretion remained constant and at a high level during the same culture period (160UI/L/mug DNA). Our data demonstrated that in addition to the ST, invasive EVCT also expressed and secreted high levels of hCG, suggesting a specific paracrine and/or autocrine role for hCG from EVCT origin.
Document type :
Journal articles
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00000052
Contributor : Danièle Evain-Brion <>
Submitted on : Friday, June 2, 2006 - 2:59:28 PM
Last modification on : Wednesday, October 14, 2020 - 4:11:06 AM
Long-term archiving on: : Monday, September 17, 2012 - 1:00:28 PM

Identifiers

Collections

Citation

Karen Handschuh, Jean Guibourdenche, Vassilis Tsatsaris, Mickaël Guesnon, Ingrid Laurendeau, et al.. Human Chorionic Gonadotropin Expression in Human Trophoblasts from Early Placenta: Comparative Study Between Villous and Extravillous Trophoblastic Cells.: hCG in invasive extravillous cytotrophoblast. Placenta, Elsevier, 2007, 28 (2-3), pp.175-184. ⟨10.1016/j.placenta.2006.01.019⟩. ⟨inserm-00000052⟩

Share

Metrics

Record views

580

Files downloads

2538