Skip to Main content Skip to Navigation
Journal articles

Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells.

Abstract : Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate fractions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. In particulate fractions, iNOS dimers were more abundant than in the cytosolic fraction. Similar observations were seen in mouse duodenum samples. These results suggest that, in epithelial cells, iNOS activity is regulated by localization-dependent processes.
Complete list of metadatas

Cited literature [37 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00000045
Contributor : Jean-Claude Sirard <>
Submitted on : Wednesday, March 29, 2006 - 2:08:00 PM
Last modification on : Tuesday, April 17, 2018 - 3:10:03 PM
Long-term archiving on: : Saturday, April 3, 2010 - 10:57:45 PM

Identifiers

Collections

Citation

Martin Rumbo, Françoise Courjault-Gautier, Frédéric Sierro, Jean-Claude Sirard, Emanuela Felley-Bosco. Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells.. FEBS Journal, Wiley, 2005, 272 (2), pp.444-53. ⟨10.1111/j.1742-4658.2004.04484.x⟩. ⟨inserm-00000045⟩

Share

Metrics

Record views

323

Files downloads

874