Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue FEBS Journal Année : 2005

Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells.

Résumé

Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate fractions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. In particulate fractions, iNOS dimers were more abundant than in the cytosolic fraction. Similar observations were seen in mouse duodenum samples. These results suggest that, in epithelial cells, iNOS activity is regulated by localization-dependent processes.
Fichier principal
Vignette du fichier
efbinos3.pdf (252.1 Ko) Télécharger le fichier
Loading...

Dates et versions

inserm-00000045 , version 1 (29-03-2006)

Identifiants

Citer

Martin Rumbo, Françoise Courjault-Gautier, Frédéric Sierro, Jean-Claude Sirard, Emanuela Felley-Bosco. Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells.. FEBS Journal, 2005, 272 (2), pp.444-53. ⟨10.1111/j.1742-4658.2004.04484.x⟩. ⟨inserm-00000045⟩

Collections

INSERM
67 Consultations
505 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More