hH-Rev107, a class II tumor suppressor gene, is expressed by post-meiotic testicular germ cells and CIS cells but not by human testicular germ cell tumors. - Archive ouverte HAL Access content directly
Journal Articles Oncogene Year : 2001

hH-Rev107, a class II tumor suppressor gene, is expressed by post-meiotic testicular germ cells and CIS cells but not by human testicular germ cell tumors.

(1) , (1) , (1) , (1) , (2) , (3) , (1) , (1)
1
2
3

Abstract

By systematic analysis of a human testis library, we have isolated the hH-Rev107-3 cDNA, identical to hH-Rev107-1 cDNA, which was previously described as a class II tumor suppressor gene. In this study, two transcripts (1 and 0.8 kb) were detected by Northern blot in all human tissues, excepted in thymus. The strongest expression was found in testis, skeletal muscle and heart. These two mRNA are probably transcribed from only one gene that we mapped to the q12-q13 region of the chromosome 11. In human testis, hH-Rev107 gene expression was localized, by in situ hybridization, within the round spermatids. To investigate a possible role for hH-Rev107 protein in testicular malignant growth, we examined the expression of this gene in germ cell tumors. A strong hH-Rev107 gene expression was observed in normal testis as well as in samples with preinvasive carcinoma in situ but was completely absent in overt tumors, both seminomas and non-seminomas. By in situ hybridization, CIS was found hH-Rev107 positive and tumor negative. A semi-quantitative assessment of hH-Rev107 mRNA level in testicular germ cell tumors, by RT-PCR, exhibited a ninefold decrease in the gene expression. No gross structural aberrations of hH-Rev107 gene were detected in these human primary tumors. The results suggest that down-regulation of hH-Rev107 may be associated with invasive progression of testicular germ cell tumors.
Fichier principal
Vignette du fichier
Siegrist_et_al.pdf (2.97 Mo) Télécharger le fichier
Loading...

Dates and versions

inserm-00000004 , version 1 (01-02-2006)

Identifiers

Cite

Sylvie Siegrist, Chloé Féral, Mounia Chami, Brigitte Solhonne, Marie-Geneviève Mattei, et al.. hH-Rev107, a class II tumor suppressor gene, is expressed by post-meiotic testicular germ cells and CIS cells but not by human testicular germ cell tumors.. Oncogene, 2001, 20 (37), pp.5155-63. ⟨10.1038/sj/onc/1204658⟩. ⟨inserm-00000004⟩

Collections

INSERM UNIV-AMU
133 View
274 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More