Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant Haberlea rhodopensis - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue International Journal of Molecular Sciences Année : 2022

Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant Haberlea rhodopensis

Diana Zasheva
  • Fonction : Auteur
  • PersonId : 1155021
Denis Falconet
Myriam Ferro
Jenny Renaut
Xin Deng
  • Fonction : Auteur
  • PersonId : 1155028

Résumé

Global warming and drought stress are expected to have a negative impact on agricultural productivity. Desiccation-tolerant species, which are able to tolerate the almost complete desiccation of their vegetative tissues, are appropriate models to study extreme drought tolerance and identify novel approaches to improve the resistance of crops to drought stress. In the present study, to better understand what makes resurrection plants extremely tolerant to drought, we performed transmission electron microscopy and integrative large-scale proteomics, including organellar and phosphorylation proteomics, and combined these investigations with previously published transcriptomic and metabolomics data from the resurrection plant Haberlea rhodopensis. The results revealed new evidence about organelle and cell preservation, posttranscriptional and posttranslational regulation, photosynthesis, primary metabolism, autophagy, and cell death in response to desiccation in H. rhodopensis. Different protective intrinsically disordered proteins, such as late embryogenesis abundant (LEA) proteins, thaumatin-like proteins (TLPs), and heat shock proteins (HSPs), were detected. We also found a constitutively abundant dehydrin in H. rhodopensis whose phosphorylation levels increased under stress in the chloroplast fraction. This integrative multi-omics analysis revealed a systemic response to desiccation in H. rhodopensis and certain targets for further genomic and evolutionary studies on DT mechanisms and genetic engineering towards the improvement of drought tolerance in crops.
Fichier principal
Vignette du fichier
Mladenov et al - Int J Mol Sci .pdf (34.14 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03747437 , version 1 (08-08-2022)

Identifiants

Citer

Petko Mladenov, Diana Zasheva, Sébastien Planchon, Céline C Leclercq, Denis Falconet, et al.. Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant Haberlea rhodopensis. International Journal of Molecular Sciences, 2022, 23 (15), pp.8520. ⟨10.3390/ijms23158520⟩. ⟨hal-03747437⟩
83 Consultations
9 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More