Skip to Main content Skip to Navigation
Journal articles

Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice

Abstract : Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role. To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet. Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine. It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition. In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine. Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium). Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
Complete list of metadata

https://hal.sorbonne-universite.fr/hal-03345382
Contributor : Gestionnaire Hal-Su Connect in order to contact the contributor
Submitted on : Wednesday, September 15, 2021 - 3:13:24 PM
Last modification on : Tuesday, September 21, 2021 - 2:32:05 PM

File

s41598-021-95117-0.pdf
Publication funded by an institution

Identifiers

`

Citation

Amélie Bravard, Céline Gérard, Clémence Defois, Bérengère Benoit, Kassem Makki, et al.. Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice. Scientific Reports, Nature Publishing Group, 2021, 11, pp.16684. ⟨10.1038/s41598-021-95117-0⟩. ⟨hal-03345382⟩

Share

Metrics

Record views

31

Files downloads

42