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Journal articles
Innovative particle standards and long-lived imaging for 2D and 3D dSTORM.
Angelina Provost
Corentin Rousset
Laura Bourdon
Sarra Mezhoud
Emma Reungoat
1
Camille Fourneaux
Timothée Bresson
Marine Pauly
Nicolas Béard
Laura Possi-Tchouanlong
Boyan Grigorov
1
Philippe Bouvet
Jean-Jacques Diaz
Christophe Chamot
Eve-Isabelle Pécheur
1
Catherine Ladaviere
Marie-Thérèse Charreyre
Christophe Place
Karine Monier
Arnaud Favier
2
Jean-Jacques Diaz
Author
Christophe Chamot
Author
Eve-Isabelle Pécheur 1
Author IdHAL : eve-isabelle-pecheur ORCID : https://orcid.org/0000-0002-8613-862X
Catherine Ladaviere
Author IdHAL : catherine-ladaviere ORCID : https://orcid.org/0000-0002-6039-7471
Marie-Thérèse Charreyre
Author
Christophe Place
Author IdHAL : christophe-place ORCID : https://orcid.org/0000-0001-7558-5013
Karine Monier
Author
Arnaud Favier 2
Author IdHAL : afavier ResearcherId : http://www.researcherid.com/rid/F-4947-2018 ORCID : https://orcid.org/0000-0002-7482-7874
1
UNICANCER/CRCL - Centre de Recherche en Cancérologie de Lyon (28, rue Laennec - 69373 Lyon - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1052 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5286 (France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
- Centre Léon Bérard [Lyon] (28, rue Laennec 69373 LYON Cedex 08 - France)
2
IMP - Ingénierie des Matériaux Polymères (17, Av. Jean Capelle 69621 Villeurbanne Cedex - France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
- INSA Lyon - Institut National des Sciences Appliquées de Lyon (20 Avenue Albert Einstein,
69621 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
- INSA - Institut National des Sciences Appliquées (France)
- UJM - Université Jean Monnet [Saint-Étienne] (10 rue Tréfilerie - 42100 Saint-Étienne - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5223 (France)
Abstract : Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking particle standards and conditions for long-lived imaging over several weeks. Stable localisation precisions within the 10 nm-range were achieved for single virions and in cellulo 2D imaging of centrosomes, as well as their reliable reconstruction in 3D dSTORM.
Document type :
Journal articles
Complete list of metadatas
https://hal.archives-ouvertes.fr/hal-03018852
Contributor : Eve-Isabelle Pecheur <>
Submitted on : Monday, November 23, 2020 - 11:53:03 AM
Last modification on : Wednesday, December 2, 2020 - 3:28:29 AM
Contributor : Eve-Isabelle Pecheur <>
Submitted on : Monday, November 23, 2020 - 11:53:03 AM
Last modification on : Wednesday, December 2, 2020 - 3:28:29 AM
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Scientific_Reports2019.pdf
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Identifiers
- HAL Id : hal-03018852, version 1
- DOI : 10.1038/s41598-019-53528-0
- PUBMED : 31784555
- PUBMEDCENTRAL : PMC6884466
Collections
UNIV-LYON1 | CRCL | CNRS | UDL | UNIV-ST-ETIENNE | INC-CNRS | INSA-LYON | INSA-GROUPE | IMP
Citation
Angelina Provost, Corentin Rousset, Laura Bourdon, Sarra Mezhoud, Emma Reungoat, et al.. Innovative particle standards and long-lived imaging for 2D and 3D dSTORM.. Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.17967. ⟨10.1038/s41598-019-53528-0⟩. ⟨hal-03018852⟩
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