Heterogeneity in sarcoma cell lines reveals enhanced motility of tetraploid versus diploid cells - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Oncotarget Année : 2017

Heterogeneity in sarcoma cell lines reveals enhanced motility of tetraploid versus diploid cells

Résumé

Soft tissue sarcomas with complex genomics are very heterogeneous tumors lacking simple prognosis markers or targeted therapies. Overexpression of a subset of mitotic genes from a signature called CINSARC is of bad prognosis, but the significance of this signature remains elusive. Here we precisely measure the cell cycle and mitosis duration of sarcoma cell lines and we found that the mitotic gene products overexpression does not reflect variation in the time spent during mitosis or G2/M. We also found that the CINSARC cell lines, we studied, are composed of a mixture of aneuploid, diploid, and tetraploid cells that are highly motile in vitro. After sorting diploid and tetraploid cells, we showed that the tetraploid cell clones do not possess a proliferative advantage, but are strikingly more motile and invasive than their diploid counterparts. This is correlated with higher levels of mitotic proteins overexpression. Owing that mitotic proteins are almost systematically degraded at the end of mitosis, we propose that it is the abnormal activity of the mitotic proteins during interphase that boosts the sarcoma cells migratory properties by affecting their cytoskeleton. To test this hypothesis, we designed a screen for mitotic or cytoskeleton protein inhibitors affecting the sarcoma cell migration potential independently of cytotoxic activities. We found that inhibition of several mitotic kinases drastically impairs the CINSARC cell invasive and migratory properties. This finding could provide a handle by which to selectively inhibit the most invasive cells.
Fichier principal
Vignette du fichier
oncotarget-08-16669.pdf (6.31 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01875251 , version 1 (09-06-2021)

Licence

Paternité

Identifiants

Citer

Mohamed Jemaà, Samer Abdallah, Gwendaline Lledo, Gaelle Perot, Tom Lesluyes, et al.. Heterogeneity in sarcoma cell lines reveals enhanced motility of tetraploid versus diploid cells. Oncotarget, 2017, 8 (10), ⟨10.18632/oncotarget.14291⟩. ⟨hal-01875251⟩
92 Consultations
23 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More