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Article Dans Une Revue Current Stem Cell Research & Therapy Année : 2017

Regenerative potential of primary adult human neural stem cells on micropatterned bioimplants boosts motor recovery

Résumé

Background: The adult brain is unable to regenerate itself sufficiently after large injuries . Therefore, hopes rely o n therapies using neural stem cell or biomaterial transplantation to sustain brain reconstruction. The aim of the present study was to evaluate the improvement in sensorimotor recovery brought about by human p rimary adult NSCs in combination with bio implant s . Methods: hNSCs were pre - seeded on implants micropatterned for neurite guidance and inserted intracerebrally 2 weeks after a primary motor cortex lesion in rats . Long - term behavio u r was significantly improved after hNSC - Implants versus cell engraf tment in the grip strength test. MRI and immunohistological studies were conducted to elucidate the underlying mechanisms of neuro implants integration . Results: hNSC - I mplants promoted tissue reconstruction and limited hemispheric atrophy and glial scar expansion. After 3 months , grafted hNSCs were detected on implants and expressed mature neuron al markers (NeuN, MAP2, and SMI 312). They also migrated over a short distance to the reconstructed and to the perilesional tissue s , where 26% integrated as mature neurons. Newly formed host neural progenitors ( n e stin, DCX) colonized the implants, notably in the presence of hNSCs, and participated to tissue reconstruction. The microstructured bioimplants sustain ed the guided maturation of both grafted hNSCs and endo genous progenitors. Conclusions: t hese immunohistological results are coherent with and could explain the late improvement observed in sensorimotor recovery. These findings provide novel insight s into the regenerative potential of primary adult hNSCs combined with microstructure d implants .

Domaines

Neurobiologie
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Dates et versions

hal-01618948 , version 1 (18-10-2017)
hal-01618948 , version 2 (12-11-2017)

Identifiants

  • HAL Id : hal-01618948 , version 1

Citer

Carole Davoust, Benjamin Plas, Amélie Béduer, Boris Demain, Anne-Sophie Salabert, et al.. Regenerative potential of primary adult human neural stem cells on micropatterned bioimplants boosts motor recovery . Current Stem Cell Research & Therapy, In press, 38p. ⟨hal-01618948v1⟩
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