Table of primary antibodies used.
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Table of primary antibodies used.
| Antigen | Immunogen | Manufacturer, species, type, catalog number | Dilution used |
|---|---|---|---|
| occludin | GST fusion protein consisting of aa 372-522 from C-terminus of human occludin fused to GST | Zymed (San Francisco, CA), rabbit polyclonal, #1379345 | 1:500 |
| zonula occludens-1 | 69 kD fusion protein corresponding to aa 463-1109 of human ZO-1 | Zymed, rabbit polyclonal, #60303915 | 1:500 |
| claudin 1 | N-CRK TTS YPT PRP YPK PAP SSG KDY V-C synthetic peptide in the C-terminal sequence of human claudin 1 | Zymed, rabbit polyclonal, #60782673 | 1:100 |
| claudin 3 | N-CPR STG PGT GTG TAY DRK DYV-C synthetic peptide in the C-terminal sequence of human claudin 3 | Zymed, rabbit polyclonal, #1376469 | 1:100 |
| claudin 5 | N-CYS APR RPT ANG DYD KKN YV – C synthetic peptide in the C-terminal sequence of mouse claudin 5 | Zymed, rabbit polyclonal, #51001292 | 1:100 |
| detyrosinated tubulin | N-GEEEGEE-C synthetic peptide corresponding to the seven C-terminal aa | Chemicon (Temecula, CA), rabbit polyclonal, #LV1422976 | 1:500 |
| vimentin | recombinant Syrian gold hamster vimentin | Chemicon, chicken polyclonal, #0604026541 | 1:2,000 |
| von Willebrand factor | von Willebrand factor purified from human plasma | Sigma (Saint Quentin Fallavier, France), rabbit polyclonal | 1:200 |
| MECA 32 | murine lymph node stroma | Gift from Pr Britta Engelhardt (Switzerland), rat monoclonal | 1:200 |