Microphotographs showing the distribution of claudin 5 and vimentin immunoreactivities in coronal sections of the tuberal region of the hypothalamus. A–B: Images showing the honeycomb pattern of immunoreactivity for claudin 5 (green) in both cuboidal ependymal cells (vimentin, red) (A) and tanycytes of the median eminence (vimentin, red) (B). C: Image showing the unorganized pattern of immunoreactivity for claudin 5 in tanycytes of the arcuate nucleus. Sections are counterstained using Hoechst (blue) to visualize cell nuclei and recognize the morphological limits of each hypothalamic structure. 3V, third ventricle. Scale bar = 20μm in C.