Morphological identification of the different ependymal cell types. A–D: Microphotographs showing the pattern of vimentin immunostaining (red) at the level of the ME (A), ARH (B), VMH (C) and DMH (D). E–H: Microphotographs showing the pattern of occludin immunostaining (green) at the level of the ME (E), ARH (F), VMH (G) and DMH (H). I: Merged picture of A and E. J: Merged picture of B and F. K: Merged picture of C and G. L: Merged picture of D and H. The three cell types identified on the ventricular surface of the third ventricle were tanycytes harboring a honeycomb pattern of occludin (empty arrows), tanycytes harboring an unorganized pattern of occludin (arrows) and cuboidal ependymal cells harboring a honeycomb pattern of occludin (empty arrowheads). The nuclei are counterstained using Hoechst (blue). M: Quantification of the basic cell types found on the ventricular surface at the ME, ARH, VMH and DMH zones. ARH, arcuate nucleus of the hypothalamus; DMH, dorsomedial nucleus of the hypothalamus; ME, median eminence; VMH, ventromedial nucleus of the hypothalamus. Scale bar = 20μm in L.