Figure 4. Purified antibodies diminished the level of the PrPC molecules in non infected N2a58 cells
(A) Cells cultured in 25-cm2 flasks in the presence of purified antibodies were serially passaged after 3, 6, 9 and 12 days. Every 3 days, antibodies were freshly added to the medium. (1) Control without treatment with the antibody; (2) Cells treated with 1 μg/ml of SAF34; (3) Cells treated with 1 μg/ml of SAF61; (4) Cells treated with 1 μg/ml of SAF34 and 1 μg/ml of SAF61. Immunoblots are probed with SAF32 antibodies as previously described. The western blot selected here, is representative of 3 independent experiments that were made. (B) Total RNA from cells treated as above was extracted and PrP mRNA was amplified by RT-PCR. The PCR products were then loaded on an agarose gel. (1) Control without treatment with the antibody; (2) Cells treated with both SAF34 and SAF61 at 1 μg/ml each.