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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Clotilde Thery 1 Kenneth Witwer 2 Elena Aikawa Maria Jose Alcaraz Johnathon Anderson Ramaroson Andriantsitohaina 3 Anna Antoniou Tanina Arab 4 Fabienne Archer 5 Georgia Atkin-Smith D Craig Ayre Jean-Marie Bach 6 Daniel Bachurski Hossein Baharvand Leonora Balaj Shawn Baldacchino Natalie Bauer Amy Baxter Mary Bebawy Carla Beckham Apolonija Bedina Zavec 7 Abderrahim Benmoussa 8 Anna Berardi Paolo Bergese Ewa Bielska Cherie Blenkiron Sylwia Bobis-Wozowicz Eric Boilard 9 Wilfrid Boireau 10 Antonella Bongiovanni Francesc Borràs Steffi Bosch 11 Chantal Boulanger 12 Xandra Breakefield Andrew Breglio Meadhbh Brennan 13 David Brigstock Alain Brisson 14 Marike Ld Broekman Jacqueline Bromberg Paulina Bryl-Górecka Shilpa Buch Amy Buck Dylan Burger Sara Busatto Dominik Buschmann Benedetta Bussolati 15 Edit Buzás James Bryan Byrd Giovanni Camussi 16 David Rf Carter Sarah Caruso Lawrence Chamley Yu-Ting Chang Chihchen Chen Shuai Chen Lesley Cheng 17 Andrew Chin 18 Aled Clayton 19 Stefano Clerici Alex Cocks Emanuele Cocucci Robert Coffey Anabela Cordeiro-Da-Silva 20 Yvonne Couch Frank Aw Coumans Beth Coyle Rossella Crescitelli Miria Ferreira Criado Crislyn D’souza-Schorey Saumya Das Amrita Datta Chaudhuri Paola de Candia Eliezer de Santana Olivier de Wever 21 Hernando del Portillo Tanguy Demaret Sarah Deville Andrew Devitt Bert Dhondt 21 Dolores Di Vizio Lothar Dieterich Vincenza Dolo Ana Paula Dominguez Rubio Massimo Dominici 22 Mauricio Dourado Tom Ap Driedonks Filipe Duarte Heather Duncan Ramon Eichenberger Karin Ekström Samir El Andaloussi 23 Céline Elie-Caille 10 Uta Erdbrügger Juan Falcón-Pérez 24 Farah Fatima Jason Fish Miguel Flores-Bellver András Försönits Annie Frelet-Barrand 10 Fabia Fricke Gregor Fuhrmann Susanne Gabrielsson Ana Gámez-Valero Chris Gardiner Kathrin Gartner 25 Raphael Gaudin Yong Song Gho Bernd Giebel 26 Caroline Gilbert 27 Mario Gimona Ilaria Giusti Deborah Ci Goberdhan André Görgens Sharon Gorski David Greening Julia Christina Gross Alice Gualerzi Gopal Gupta Dakota Gustafson Aase Handberg Reka Haraszti Paul Harrison 28 Hargita Hegyesi An Hendrix 21 Andrew Hill Fred Hochberg Karl Hoffmann 29 Beth Holder Harry Holthöfer 30 Baharak Hosseinkhani Guoku Hu Yiyao Huang Veronica Huber Stuart Hunt Ahmed Gamal-Eldin Ibrahim Tsuneya Ikezu Jameel Inal Mustafa Isin Alena Ivanova Hannah Jackson Soren Jacobsen 31 Steven Jay Muthuvel Jayachandran Guido Jenster Lanzhou Jiang Suzanne Johnson Jennifer Jones 32 Ambrose Jong Tijana Jovanovic-Talisman Stephanie Jung Raghu Kalluri Shin-Ichi Kano Sukhbir Kaur Yumi Kawamura Evan Keller Delaram Khamari Elena Khomyakova Anastasia Khvorova Peter Kierulf Kwang Pyo Kim Thomas Kislinger Mikael Klingeborn David Klinke Miroslaw Kornek Maja Kosanović Árpád Ferenc Kovács Eva-Maria Krämer-Albers 33 Susanne Krasemann Mirja Krause Igor Kurochkin Gina Kusuma Sören Kuypers Saara Laitinen Scott Langevin Lucia Languino Joanne Lannigan Cecilia Lässer Louise Laurent Grégory Lavieu 34 Elisa Lázaro-Ibáñez Soazig Le Lay 3 Myung-Shin Lee Yi Xin Fiona Lee Debora Lemos Metka Lenassi Aleksandra Leszczynska Isaac Ts Li Ke Liao Sten Libregts Erzsebet Ligeti Rebecca Lim Sai Kiang Lim Aija Linē Karen Linnemannstöns Alicia Llorente Catherine Lombard Magdalena Lorenowicz Ákos Lörincz Jan Lötvall 35 Jason Lovett Michelle Lowry Xavier Loyer 12 Quan Lu Barbara Lukomska Taral Lunavat Sybren Ln Maas Harmeet Malhi Antonio Marcilla 36 Jacopo Mariani Javier Mariscal Elena Martens-Uzunova Lorena Martin-Jaular M Carmen Martinez 3 Vilma Regina Martins Mathilde Mathieu 37 Suresh Mathivanan 17 Marco Maugeri Lynda Mcginnis Mark Mcvey David Meckes Katie Meehan Inge Mertens 38 Valentina Minciacchi Andreas Möller Malene Møller Jørgensen Aizea Morales-Kastresana Jess Morhayim François Mullier 39 Maurizio Muraca 40 Luca Musante Veronika Mussack Dillon Muth Kathryn Myburgh Tanbir Najrana Muhammad Nawaz Irina Nazarenko Peter Nejsum 41 Christian Neri 42 Tommaso Neri Rienk Nieuwland Leonardo Nimrichter John Nolan 43 Esther Nm Nolte-’t Hoen Nicole Noren Hooten Lorraine O’driscoll Tina O’grady Ana O’loghlen Takahiro Ochiya Martin Olivier 44 Alberto Ortiz 45 Luis Ortiz 46 Xabier Osteikoetxea Ole Østergaard Matias Ostrowski Jaesung Park D. Michiel Pegtel Héctor Peinado 47 Francesca Perut 48 Michael Pfaffl Donald Phinney 49 Bartijn Ch Pieters Ryan Pink David Pisetsky Elke Pogge von Strandmann Iva Polakovicova Ivan Kh Poon Bonita Powell Ilaria Prada Lynn Pulliam Peter Quesenberry Annalisa Radeghieri Robert Raffai Stefania Raimondo 50 Janusz Rak 51 Marcel Ramirez Graça Raposo 52 Morsi Rayyan Neta Regev-Rudzki Franz Ricklefs Paul Robbins David Roberts 53 Silvia Rodrigues Eva Rohde Sophie Rome 54 Kasper Ma Rouschop Aurelia Rughetti Ashley Russell Paula Saá Susmita Sahoo 55 Edison Salas-Huenuleo Catherine Sánchez Julie Saugstad Meike Saul Raymond Schiffelers 56 Raphael Schneider 57 Tine Hiorth Schøyen Aaron Scott Eriomina Shahaj Shivani Sharma Olga Shatnyeva Faezeh Shekari Ganesh Vilas Shelke Ashok Shetty Kiyotaka Shiba Pia R-M Siljander Andreia Silva Agata Skowronek Orman Snyder Rodrigo Pedro Soares 58 Barbara Sódar Carolina Soekmadji Javier Sotillo Philip Stahl Willem Stoorvogel Shannon Stott Erwin Strasser Simon Swift Hidetoshi Tahara Muneesh Tewari Kate Timms Swasti Tiwari Rochelle Tixeira Mercedes Tkach Wei Seong Toh Richard Tomasini 59 Ana Cláudia Torrecilhas 60 Juan Pablo Tosar 61 Vasilis Toxavidis Lorena Urbanelli 62 Pieter Vader Bas Wm van Balkom Susanne van der Grein 63 Jan van Deun 21 Martijn Jc van Herwijnen Kendall van Keuren-Jensen Guillaume van Niel 64 Martin van Royen Andre van Wijnen M Helena Vasconcelos Ivan Vechetti Tiago Veit Laura Vella Emilie Velot 65 Frederik Verweij 64 Beate Vestad Jose Viñas Tamás Visnovitz Krisztina Vukman Jessica Wahlgren Dionysios Watson Marca Hm Wauben Alissa Weaver Jason Webber Viktoria Weber Ann Wehman 66 Daniel Weiss 67 Joshua Welsh Sebastian Wendt Asa Wheelock Zoltán Wiener Leonie Witte Joy Wolfram Angeliki Xagorari Patricia Xander Jing Xu 68 Xiaomei Yan 69 María Yáñez-Mó Hang Yin 70 Yuana Yuana Valentina Zappulli Jana Zarubova Vytautas Žėkas Jian-Ye Zhang Zezhou Zhao Lei Zheng Alexander Zheutlin Antje Zickler 71 Pascale Zimmermann 59 Angela Zivkovic Davide Zocco Ewa Zuba-Surma
42 Brain-C - Biologie et Pathologie du Neurone
B2A - Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing
Abstract : The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
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https://hal.archives-ouvertes.fr/hal-02323217
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Clotilde Thery, Kenneth Witwer, Elena Aikawa, Maria Jose Alcaraz, Johnathon Anderson, et al.. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles, Taylor & Francis, 2018, 7 (1), pp.1535750. ⟨10.1080/20013078.2018.1535750⟩. ⟨hal-02323217⟩

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