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Extraction and sequencing of single nuclei from murine skeletal muscles

Abstract : Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows us to sequence the myonuclei of the multinucleated myofibers. Second, it circumvents the cell-dissociation-induced transcriptional modifications. For complete details on the use and execution of this protocol, please refer to Dos Santos et al. (2020) and Machado, Geara et al. (2021).
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https://hal.archives-ouvertes.fr/hal-03376200
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Submitted on : Wednesday, October 13, 2021 - 12:57:03 PM
Last modification on : Tuesday, October 19, 2021 - 4:10:33 PM

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Matthieu Dos Santos, Stamatia Gioftsidi, Stéphanie Backer, Léo Machado, Frederic Relaix, et al.. Extraction and sequencing of single nuclei from murine skeletal muscles. STAR Protocols, Elsevier, 2021, 2 (3), pp.100694. ⟨10.1016/j.xpro.2021.100694⟩. ⟨hal-03376200⟩

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