An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures

Marie Bidart; Isabelle Bonnet; Aurélie Hennebique; Zine Eddine Kherraf; Hervé Pelloux; François Berger; Muriel Cornet; Sébastien Bailly; Danièle Maubon

doi:10.1128/JCM.03600-14

Journal articles

An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures

Marie Bidart ¹ Isabelle Bonnet ¹ Aurélie Hennebique ¹ Zine Eddine Kherraf ¹ Hervé Pelloux ¹ François Berger ¹ Muriel Cornet ^{1, 2} Sébastien Bailly ^{1, 3} Danièle Maubon ^{1, 2}

1 CHU - Centre Hospitalier Universitaire [Grenoble]

2 TIMC-TheREx - Thérapeutique Recombinante Expérimentale

TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525

3 IAME (UMR_S_1137 / U1137) - Infection, Anti-microbiens, Modélisation, Evolution

Abstract : We developed an in-house assay for the direct identification, by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, of yeasts in blood culture. Sixty-one representative strains from 12 species were analyzed in spiked blood cultures. Our assay accurately identified 95 of 107 (88.8%) positive blood cultures and outperformed the commercial Sepsityper kit (81.7% identification).

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology / Mycology

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https://hal.archives-ouvertes.fr/hal-03034336
Contributor : Aurélie Hennebique <>
Submitted on : Friday, January 22, 2021 - 1:52:42 PM
Last modification on : Monday, January 25, 2021 - 4:14:12 PM

An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures

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An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures

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