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Chapitre D'ouvrage Année : 2022

Measuring the NQO2: Melatonin Complex by Native Nano-Electrospray Ionization Mass Spectrometry

Résumé

Melatonin exerts its effects through a series of target proteins/receptors and enzymes. Its antioxidant capacity might be due to its capacity to inhibit a quinone reductase (NQO2) at high concentration (50 µM). Demonstrating the existence of a complex between a compound and a protein is often not easy. It requires either that the compound is an inhibitor-and the complex translates by an inhibition of the catalytic activity-or the compound is radiolabeled-and the complex translates in standard binding approaches, such as in receptology. Outside these two cases, the detection of the protein:small molecule complexes by mass spectrometry has recently been made possible, thanks to the development of so called native mass spectrometry. Using this approach one can measure masses corresponding to a intact noncovalent complex between a compound and its target, usually after titration or competition experiments. In the present chapter, we detail the characterization of NQO2:melatonin interaction using native mass spectrometry .

Domaines

Chimie
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Dates et versions

hal-03843794 , version 1 (08-11-2022)

Identifiants

Citer

Jean Boutin, Johann Stojko, Gilles Ferry, Sarah Cianférani. Measuring the NQO2: Melatonin Complex by Native Nano-Electrospray Ionization Mass Spectrometry. Melatonin, 2550, Springer US, pp.323-328, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2593-4_34⟩. ⟨hal-03843794⟩
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