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Article Dans Une Revue Scientific Reports Année : 2015

An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E

Résumé

The enzymatic activity of the pathogenic botulinum neurotoxins type A and E (BoNT/A and E) leads to potentially lethal paralytic symptoms in humans and their prompt detection is of crucial importance. A chip assay based on Surface Plasmon Resonance monitoring of the cleavage products is a simple method that we have previously established to detect BoNT/A activity. We have now developed a similar format assay to measure BoNT/E activity. A monoclonal antibody specifically recognizing SNAP25 cleaved by BoNT/E was generated and used to measure the appearance of the neo-epitope following injection of BoNT/E over SNAP-25 immobilized on a chip. This assay detects BoNT/E activity at 1 LD50/ml within minutes and linear dose-responses curves were obtained using a multiplexed biosensor. A threshold of 0.01 LD50/ml was achieved after 5 h of cleavage. This assay is 10-fold more sensitive than the in vivo assay for direct detection of BoNT/E in serum samples. The SNAP25 chip assay is able to discriminate in an automated manner the presence of BoNT/E, BoNT/A or a combination of both toxins.
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inserm-01708826 , version 1 (19-02-2018)

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Christian Lévêque, Géraldine Ferracci, Yves Maulet, Christelle Mazuet, Michel Popoff, et al.. An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E. Scientific Reports, 2015, 5 (1), pp.17953. ⟨10.1038/srep17953⟩. ⟨inserm-01708826⟩
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