Syndecan-1 regulates the biological activities of interleukin-34: Syndecan-1 is a co-receptor for IL-34

Abstract : IL-34 is a challenging cytokine sharing functional similarities with M-CSF through M-CSFR activation. It also plays a singular role that has recently been explained in the brain, through a binding to the receptor protein tyrosine phosphatase RPTPβ/ζ. The aim of this paper was to look for alternative binding of IL-34 on other cell types. Myeloid cells (HL-60, U-937, THP-1) were used as cells intrinsically expressing M-CSFR, and M-CSFR was expressed in TF-1 and HEK293 cells. IL-34 binding was studied by Scatchard and binding inhibition assays, using 125I-radiolabelled cytokines, and surface plasmon resonance. M-CSFR activation was analysed by Western blot after glycosaminoglycans abrasion, syndecan-1 overexpression or repression and addition of a blocking anti-syndecan antibody. M-CSF and IL-34 induced different patterns of M-CSFR phosphorylations, suggesting the existence of alternative binding for IL-34. Binding experiments and chondroitinase treatment confirmed low affinity binding to chondroitin sulphate chains on cells lacking both M-CSFR and RPTPβ/ζ. Amongst the proteoglycans with chondroitin sulphate chains, syndecan-1 was able to modulate the IL-34-induced M-CSFR signalling pathways. Interestingly, IL-34 induced the migration of syndecan-1 expressing cells. Indeed, IL-34 significantly increased the migration of THP-1 and M2a macrophages that was inhibited by addition of a blocking anti-syndecan-1 antibody. This paper provides evidence of alternative binding of IL-34 to chondroitin sulphates and syndecan-1 at the cell surface that modulates M-CSFR activation. In addition, IL-34-induced myeloid cell migration is a syndecan-1 dependent mechanism.
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BBA - Biochimica et Biophysica Acta, Elsevier, 2015, 1853 (5), pp.1010 - 1021. 〈10.1016/j.bbamcr.2015.01.023〉
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Dernière modification le : jeudi 5 avril 2018 - 10:36:48

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Aude Segaliny, Régis Brion, Erwan Mortier, Mike Maillasson, Michel Chérel, et al.. Syndecan-1 regulates the biological activities of interleukin-34: Syndecan-1 is a co-receptor for IL-34. BBA - Biochimica et Biophysica Acta, Elsevier, 2015, 1853 (5), pp.1010 - 1021. 〈10.1016/j.bbamcr.2015.01.023〉. 〈inserm-01644783〉

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