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S. Figure, Comparison between the Micropipette Force Probe (MFP) and the Biomembrane Force Probe (BFP) The pulling loading rate was measured for the activation of human primary CD4+ T cells with anti-CD3/anti-CD28 beads (MFP) and anti-CD3 beads (BFP)

J. Husson, K. Chemin, A. Bohineust, C. Hivroz, H. et al., Force Generation upon T Cell Receptor Engagement. PLoS One 6, e19680.), the MFP data are in Figure 3C. Each data point shows mean±s.d. over one day of experiments, The BFP data was published before, 2011.

S. Figure, Human primary CD4+ T cells were pre-incubated for 15 minutes at 37°C with 30 ?M ML-7, the MLCK inhibitor, or the vehicle (DMSO) alone. Cells were then activated for 10 to 30 minutes with anti-CD3/anti-CD28 beads in the presence of ML-7. Phosphorylation of the MLC was measured by Western blot analysis. The gp96 protein in each lane is shown as a loading control, Compared to control (DMSO), the cells incubated with ML-7 show less phosphorylation of MLC demonstrating that ML-7 is inhibiting MLCK in our conditions