Neuroscience in the third dimension: shedding new light on the brain with tissue clearing

Robin Vigouroux 1 Morgane Belle 1 Alain Chédotal 1, *
* Auteur correspondant
Abstract : AbstractFor centuries analyses of tissues have depended on sectioning methods. Recent developments of tissue clearing techniques have now opened a segway from studying tissues in 2 dimensions to 3 dimensions. This particular advantage echoes heavily in the field of neuroscience, where in the last several years there has been an active shift towards understanding the complex orchestration of neural circuits. In the past five years, many tissue-clearing protocols have spawned. This is due to varying strength of each clearing protocol to specific applications. However, two main protocols have shown their applicability to a vast number of applications and thus are exponentially being used by a growing number of laboratories. In this review, we focus specifically on two major tissue-clearing method families, derived from the 3DISCO and the CLARITY clearing protocols. Moreover, we provide a “hands-on” description of each tissue clearing protocol and the steps to look out for when deciding to choose a specific tissue clearing protocol. Lastly, we provide perspectives for the development of tissue clearing protocols into the research community in the fields of embryology and cancer.
Type de document :
Article dans une revue
Molecular Brain, BioMed Central, 2017, 10 (1), pp.33. 〈10.1186/s13041-017-0314-y〉
Liste complète des métadonnées

Littérature citée [45 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01567366
Contributeur : Bmc Bmc <>
Soumis le : dimanche 23 juillet 2017 - 05:15:08
Dernière modification le : jeudi 11 janvier 2018 - 06:22:25

Fichier

13041_2017_Article_314.pdf
Publication financée par une institution

Identifiants

Collections

Citation

Robin Vigouroux, Morgane Belle, Alain Chédotal. Neuroscience in the third dimension: shedding new light on the brain with tissue clearing. Molecular Brain, BioMed Central, 2017, 10 (1), pp.33. 〈10.1186/s13041-017-0314-y〉. 〈inserm-01567366〉

Partager

Métriques

Consultations de la notice

64

Téléchargements de fichiers

36