Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro

Jérome Chal 1, 2, 3, 4 Ziad Al Tanoury 3 Marie Hestin 2, 4, 1 Bénédicte Gobert 3 Suvi Aivio 2, 4, 1 Aurore Hick 5 Thomas Cherrier 3 Alexander Nesmith 6 Kevin Parker 6 Olivier Pourquié 2, 3, 4, 1, *
* Auteur correspondant
1 Harvard Stem Cell Institute [Cambridge, MA, USA]
2 Department of Genetics [Boston]
3 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
4 Department of Pathology [Boston, MA, USA]
5 Anagenesis Biotechnologies [Illkirch Graffenstaden]
6 Disease Biophysics Group [Cambridge, MA, USA]
Abstract : Progress toward finding a cure for muscle diseases has been slow because of the absence of relevant cellular models and the lack of a reliable source of muscle progenitors for biomedical investigation. Here we report an optimized serum-free differentiation protocol to efficiently produce striated, millimeter-long muscle fibers together with satellite-like cells from human pluripotent stem cells (hPSCs) in vitro. By mimicking key signaling events leading to muscle formation in the embryo, in particular the dual modulation of Wnt and bone morphogenetic protein (BMP) pathway signaling, this directed differentiation protocol avoids the requirement for genetic modifications or cell sorting. Robust myogenesis can be achieved in vitro within 1 month by personnel experienced in hPSC culture. The differentiating culture can be subcultured to produce large amounts of myogenic progenitors amenable to numerous downstream applications. Beyond the study of myogenesis, this differentiation method offers an attractive platform for the development of relevant in vitro models of muscle dystrophies and drug screening strategies, as well as providing a source of cells for tissue engineering and cell therapy approaches.
Keywords : pluripotent stem cells myogenesis skeletal muscle satellite cell bioengineering directed differentiation embryonic stem cell iPS Paraxial mesoderm
Type de document :
Article dans une revue
Nature Protocols, Nature Publishing Group, 2016, 11 (10), pp.1833-50. 〈10.1038/nprot.2016.110〉
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http://www.hal.inserm.fr/inserm-01485521
Contributeur : Jerome Chal <>
Soumis le : mercredi 8 mars 2017 - 23:15:54
Dernière modification le : jeudi 15 mars 2018 - 01:33:52
Document(s) archivé(s) le : vendredi 9 juin 2017 - 14:24:24

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Jérome Chal, Ziad Al Tanoury, Marie Hestin, Bénédicte Gobert, Suvi Aivio, et al.. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nature Protocols, Nature Publishing Group, 2016, 11 (10), pp.1833-50. 〈10.1038/nprot.2016.110〉. 〈inserm-01485521〉

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