Thermosensitivity profile of malign glioma U87-MG cells and human endothelial cells following γ-Fe2O3 NPs internalization and magnetic field application

Abstract : In this study we evaluate the thermosensitivity of healthy endothelial cells (HUVEC) and malign gioblastom (U87-MG) to magnetic hyperthermia (ac-magnetic field of 700 kHz, 23.10 kA.m-1) for 1 hour with and without the presence of superparamagnetic 10 nm sized polyol-made γ-Fe2O3 nanoparticles (NPs). Interestingly, despite their reduced size, NPs exhibit a high magnetization, close to that of the bulk material, in relation with their high crystalline quality. In practice, they ensured an efficient heating capacity, leading to about 20 % and more than 50 % cell death of HUVEC and U87-MG lines, respectively, when hyperthermia assays were achieved in presence of these NPs. Magnetophoresis and X-Ray fluorescence spectrometry measurements evidenced a more important internalization of NPs in U87-MG than in HUVECs. Surprisingly both cell lines reached the same maximal temperature, namely 42°C, after hyperthermia treatment suggesting a higher thermosensitivity of the former compared to the latter, establishing the fact that polol-madeγ-Fe2O3 NPs assisted hyperthermia is a harmful agent to glioma treatment.
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RSC Advances, Royal Society of Chemistry, 2016, 00, pp.1 - 3
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A. Hanini, L. Lartigue, Julie Gavard, A. Schmitt, K. Kacem, et al.. Thermosensitivity profile of malign glioma U87-MG cells and human endothelial cells following γ-Fe2O3 NPs internalization and magnetic field application. RSC Advances, Royal Society of Chemistry, 2016, 00, pp.1 - 3. 〈inserm-01406375〉

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