The E3 ubiquitin ligase MARCH3 controls the endothelial barrier - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2016

The E3 ubiquitin ligase MARCH3 controls the endothelial barrier

Résumé

Cell-cell contacts coordinate the endothelial barrier function in response to external cues. To identify new mediators involved in cytokine-promoted endothelial permeability, we screened a siRNA library targeting E3 ubiquitin ligases. Here, we report that silencing of the late endosome/lysosomal membrane-associated RING-CH-3 (MARCH3) enzyme protects the endothelial barrier. Furthermore, transcriptome analysis unmasked the upregulation of the tight junction-encoding gene occludin (OCLN) in MARCH3-depleted cells. Indeed, MARCH3 silencing results in the strengthening of cell-cell contacts, as evidenced by the accumulation of junctional proteins. From a molecular standpoint, the FoxO1 forkhead transcription repressor was inactivated in the absence of MARCH3. This provides a possible molecular link between MARCH3 and the signaling pathway involved in regulating the expression of junctional proteins and barrier integrity.

Domaines

Cancer
Fichier principal
Vignette du fichier
2016-Leclair-FEBSLettEqu15-2.pdf (820.91 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

inserm-01385212 , version 1 (21-10-2016)

Identifiants

Citer

Héloïse M Leclair, Gwennan André-Grégoire, Lucas Treps, Sandy Azzi, Nicolas Bidère, et al.. The E3 ubiquitin ligase MARCH3 controls the endothelial barrier. FEBS Letters, 2016, 590 (20), pp.3660-3668. ⟨10.1002/1873-3468.12417⟩. ⟨inserm-01385212⟩
95 Consultations
157 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More