Neuronal fate specification by the Dbx1 transcription factor is linked to the evolutionary acquisition of a novel functional domain

Abstract : AbstractBackgroundDbx1 is a homeodomain transcription factor involved in neuronal fate specification belonging to a widely conserved family among bilaterians. In mammals, Dbx1 was proposed to act as a transcriptional repressor by interacting with the Groucho corepressors to allow the specification of neurons involved in essential biological functions such as locomotion or breathing.ResultsSequence alignments of Dbx1 proteins from different species allowed us to identify two conserved domains related to the Groucho-dependent Engrailed repressor domain (RD), as well as a newly described domain composed of clusterized acidic residues at the C-terminus (Cter) which is present in tetrapods but also several invertebrates. Using a heterologous luciferase assay, we showed that the two putative repressor domains behave as such in a Groucho-dependent manner, whereas the Cter does not bear any intrinsic transcriptional activity. Consistently with in vitro data, we found that both RDs are involved in cell fate specification using in vivo electroporation experiments in the chick spinal cord. Surprisingly, we show that the Cter domain is required for Dbx1 function in vivo, acting as a modulator of its repressive activity and/or imparting specificity.ConclusionOur results strongly suggest that the presence of a Cter domain among tetrapods is essential for Dbx1 to regulate neuronal diversity and, in turn, nervous system complexity.
Type de document :
Article dans une revue
EvoDevo, 2016, 7 (1), pp.18. 〈10.1186/s13227-016-0055-5〉
Liste complète des métadonnées

Littérature citée [43 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01353739
Contributeur : Bmc Bmc <>
Soumis le : vendredi 12 août 2016 - 18:04:06
Dernière modification le : jeudi 11 janvier 2018 - 06:18:16
Document(s) archivé(s) le : dimanche 13 novembre 2016 - 11:05:12

Fichiers

13227_2016_Article_55.pdf
Publication financée par une institution

Identifiants

Collections

IJM | USPC

Citation

Sonia Karaz, Maximilien Courgeon, Hélène Lepetit, Eugenia Bruno, Raimondo Pannone, et al.. Neuronal fate specification by the Dbx1 transcription factor is linked to the evolutionary acquisition of a novel functional domain. EvoDevo, 2016, 7 (1), pp.18. 〈10.1186/s13227-016-0055-5〉. 〈inserm-01353739〉

Partager

Métriques

Consultations de la notice

150

Téléchargements de fichiers

88