Acute activation of cannabinoid receptors by anandamide reduces gastrointestinal motility and improves postprandial glycemia in mice.

Abstract : The endocannabinoid system (ECS) is associated with an alteration of glucose homeostasis dependent on cannabinoid receptor-1 (CB1R) activation. However, very little information is available concerning the consequences of ECS activation on intestinal glucose absorption. Mice were injected intraperitoneally with anandamide, an endocannabinoid binding both CB1R and CB2R. We measured plasma glucose and xylose appearance after oral loading, gastrointestinal motility, and glucose transepithelial transport using the everted sac method. Anandamide improved hyperglycemia after oral glucose charge whereas glucose clearance and insulin sensitivity were impaired, pointing out some gastrointestinal events. Plasma xylose appearance was delayed in association with a strong decrease in gastrointestinal transit, while anandamide did not alter transporter-mediated glucose absorption. Interestingly, transit was nearly normalized by coinjection of SR141716 and AM630 (CB1R and CB2R antagonist, respectively), and AM630 also reduced the delay of plasma glucose appearance induced by anandamide. When gastric emptying was bypassed by direct glucose administration in the duodenum, anandamide still reduced plasma glucose appearance in wild-type but not in CB1R(-/-) mice. In conclusion, our findings demonstrated that acute activation of intestinal ECS reduced postprandial glycemia independently on intestinal glucose transport but rather inhibiting gastric emptying and small intestine motility and strongly suggest the involvement of both CB1R and CB2R.
Type de document :
Article dans une revue
Diabetes, American Diabetes Association, 2015, 64 (3), pp.808-18. 〈10.2337/db14-0721〉
Liste complète des métadonnées

Littérature citée [48 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01344501
Contributeur : Pascal Degrace <>
Soumis le : mardi 12 juillet 2016 - 09:41:13
Dernière modification le : vendredi 8 juin 2018 - 14:50:12

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

Stephanie Troy-Fioramonti, Laurent Demizieux, Joseph Gresti, Tania Muller, Bruno Vergès, et al.. Acute activation of cannabinoid receptors by anandamide reduces gastrointestinal motility and improves postprandial glycemia in mice.. Diabetes, American Diabetes Association, 2015, 64 (3), pp.808-18. 〈10.2337/db14-0721〉. 〈inserm-01344501〉

Partager

Métriques

Consultations de la notice

66