Immature human dendritic cells enhance their migration through KCa3.1 channel activation

Abstract : Migration capacity is essential for dendritic cells (DCs) to present antigen to T cells for the induction of immune response. The DC migration is supposed to be a calcium-dependent process, while not fully understood. Here, we report a role of the KCa3.1/IK1/SK4 channels in the migration capacity of both immature (iDC) and mature (mDC) human CD14 +-derived DCs. KCa3.1 channels were shown to control the membrane potential of human DC and the Ca 2+ entry, which is directly related to migration capacities. The expression of migration marker such as CCR5 and CCR7 was modified in both types of DCs by TRAM-34 (100 nM). But, only the migration of iDC was decreased by use of both TRAM-34 and KCa3.1 siRNA. Confocal analyses showed a close localization of CCR5 with KCa3.1 in the steady state of iDC. Finally, the implication of KCa3.1 seems to be limited to the migration capacities as T cell activation of DCs appeared unchanged. Altogether, these results demonstrated that KCa3.1 channels have a pro-migratory effect on iDC migration. Our findings suggest that KCa3.1 in human iDC play a major role in their migration and constitute an attractive target for the cell therapy optimization.
Type de document :
Article dans une revue
Cell Calcium, Elsevier, 2016, 〈10.1016/j.ceca.2016.02.008〉
Liste complète des métadonnées

Littérature citée [57 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01318497
Contributeur : Florence Velge-Roussel <>
Soumis le : jeudi 19 mai 2016 - 16:12:12
Dernière modification le : vendredi 23 mars 2018 - 10:20:09

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

David Crottès, Romain Félix, Daniel Meley, Stéphanie Chadet, Florence Herr, et al.. Immature human dendritic cells enhance their migration through KCa3.1 channel activation. Cell Calcium, Elsevier, 2016, 〈10.1016/j.ceca.2016.02.008〉. 〈inserm-01318497〉

Partager

Métriques

Consultations de la notice

128