Structural and Mechanical Heterogeneity of the Erythrocyte Membrane Reveals Hallmarks of Membrane Stability

Abstract : A n essential feature of red blood cells (RBCs) relies on their capability to undergo large passive deformations throughout narrow capillaries of the micro-vasculature during their 120-days life span. Mature erythrocytes are non-nucleated, and their structural integrity depends on the particular attributes of the plasma membrane in terms of antigenic and transport activity but importantly, on its remarkable mechanical properties. 1 Many studies have unraveled the complexity hidden in the exceptional properties of the erythrocyte plasma membrane thanks to the development of isolation methods and techniques for the biochemical, structural, and functional characterization of the diverse components that integrate the erythrocyte membrane. 1À5 The RBC membrane consists of an R and β-spectrin lattice that is linked to the lipid bilayer thought membrane proteins, most of them integrating multiprotein complexes. The most abundant membrane proteins are band 3, aquaporin-1, glycophorin A and C, and the rhesus proteins, Rh and RhAG. Key for the structural integrity of the membrane are two macromolecular complexes: the an-kyrin-based complex, which is thought to work as a metabolon, and the 4.1R complex that integrates the main components of the skeleton network junctions, i.e. spectrin, actin, and protein 4.1R, together with other proteins (adducin, tropomyosin, topo-modulin, p55, and demantin) and membrane proteins such as glycophorin C (GPC) or band 3 (for a review see ref 1). It is well established, that far from being static, the RBC membrane is a metabolically regulated active structure. 5 Thus, a number of works have shown the regulatory mechanism of Ca 2þ , phosphatidylinositol 4,5-bisphos-phate (PIP 2), or protein phosphorylation on the membrane. As a result, posttranslational modifications of proteins integrating the * Address correspondence to simon.scheuring@inserm.fr.
Type de document :
Article dans une revue
ACS Nano, American Chemical Society, 2013, 7 (2), pp.1054-1063. 〈10.1021/nn303824j〉
Liste complète des métadonnées

Littérature citée [16 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01309087
Contributeur : Laboratoire U1006 Aix-Marseille Université <>
Soumis le : lundi 2 mai 2016 - 09:40:44
Dernière modification le : jeudi 17 mai 2018 - 17:18:02
Document(s) archivé(s) le : mardi 24 mai 2016 - 16:58:23

Fichier

Manuscript_Picas et al_revised...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Laura Picas, Felix Rico, Maxime Deforet, Simon Scheuring. Structural and Mechanical Heterogeneity of the Erythrocyte Membrane Reveals Hallmarks of Membrane Stability. ACS Nano, American Chemical Society, 2013, 7 (2), pp.1054-1063. 〈10.1021/nn303824j〉. 〈inserm-01309087〉

Partager

Métriques

Consultations de la notice

264

Téléchargements de fichiers

157