High-speed force spectroscopy unfolds titin at the velocity of molecular dynamics simulations.: Experiment meets simulation: HS-FS

Felix Rico 1 Laura Gonzalez 2 Ignacio Casuso 1 Manel Puig-Vidal 2 Simon Scheuring 1
1 Bio-AFM-Lab - BIO-AFM-LAB Bio Atomic Force Microscopy Laboratory
2 Bioelectronics Group, Department of Electronics
Abstract : The mechanical unfolding of the muscle protein titin by atomic force microscopy was a landmark in our understanding of single-biomolecule mechanics. Molecular dynamics simulations offered atomic-level descriptions of the forced unfolding. However, experiment and simulation could not be directly compared because they differed in pulling velocity by orders of magnitude. We have developed high-speed force spectroscopy to unfold titin at velocities reached by simulation (~4 millimeters per second). We found that a small β-strand pair of an immunoglobulin domain dynamically unfolds and refolds, buffering pulling forces up to ~100 piconewtons. The distance to the unfolding transition barrier is larger than previously estimated but is in better agreement with atomistic predictions. The ability to directly compare experiment and simulation is likely to be important in studies of biomechanical processes.
Keywords : single molecules high-speed atomic force microscopy force spectroscopy
Type de document :
Article dans une revue
Science, American Association for the Advancement of Science, 2013, 342 (6159), pp.741-3. 〈10.1126/science.1239764〉
Liste complète des métadonnées

Littérature citée [29 références]  Voir  Masquer  Télécharger
http://www.hal.inserm.fr/inserm-01309044
Contributeur : Laboratoire U1006 Aix-Marseille Université <>
Soumis le : jeudi 28 avril 2016 - 18:16:01
Dernière modification le : jeudi 17 mai 2018 - 17:18:01
Document(s) archivé(s) le : mardi 15 novembre 2016 - 16:22:33

Fichier

1239764Revisedtext_HAL.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

UNIV-AMU

Citation

Felix Rico, Laura Gonzalez, Ignacio Casuso, Manel Puig-Vidal, Simon Scheuring. High-speed force spectroscopy unfolds titin at the velocity of molecular dynamics simulations.: Experiment meets simulation: HS-FS. Science, American Association for the Advancement of Science, 2013, 342 (6159), pp.741-3. 〈10.1126/science.1239764〉. 〈inserm-01309044〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

145

Téléchargements de fichiers

172

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD