Fuse or die: how to survive the loss of Dam in Vibrio cholerae

Abstract : Dam methylates GATC sequences in γ-proteobacteria genomes, regulating several cellular functions including replication. In Vibrio cholerae, which has two chromosomes , Dam is essential for viability, owing to its role in chr2 replication initiation. In this study, we isolated spontaneous mutants of V. cholerae that were able to survive the deletion of dam. In these mutants, homologous recombination and chromosome dimer resolution are essential, unless DNA mismatch repair is inactivated. Furthermore, the initiator of chr2 repli-cation, RctB, is no longer required. We show that, instead, replication of chr2 is insured by spontaneous fusion with chr1 and piggybacking its replication machinery. We report that natural fusion of chr1 and chr2 occurred by two distinct recombination pathways: homologous recombination between repeated IS elements and site-specific recombination between dif sites. Lastly, we observed a preferential fusion of the two chromosomes in their terminus of replication.
Type de document :
Article dans une revue
Molecular Microbiology, Wiley, 2014, 〈10.1111/mmi.12483〉
Liste complète des métadonnées

Littérature citée [41 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01285641
Contributeur : Marie-Eve Val-Kennedy <>
Soumis le : mercredi 9 mars 2016 - 15:18:33
Dernière modification le : jeudi 1 février 2018 - 01:32:40
Document(s) archivé(s) le : dimanche 13 novembre 2016 - 13:12:24

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

Marie-Eve Val, Sean Patrick Kennedy, Alfonso Soler-Bistué, Valérie Barbe, Christiane Bouchier, et al.. Fuse or die: how to survive the loss of Dam in Vibrio cholerae. Molecular Microbiology, Wiley, 2014, 〈10.1111/mmi.12483〉. 〈inserm-01285641〉

Partager

Métriques

Consultations de la notice

208