Glycoxidized HDL, HDL enriched with oxidized phospholipids and HDL from diabetic patients inhibit platelet function

Abstract : CONTEXT:High-density lipoproteins (HDL) possess atheroprotective properties including anti-thrombotic and antioxidant effects. Very few studies relate to the functional effects of oxidized HDL on platelets in type 2 diabetes (T2D).OBJECTIVE:The objective of our study was to investigate the effects of in vitro glycoxidized HDL, and HDL from T2D patients on platelet aggregation and arachidonic acid signaling cascade. At the same time, the contents of hydroxylated fatty acids were assessed in HDL.RESULTS:Compared to control HDL, in vitro glycoxidized HDL had decreased proportions of linoleic (LA) and arachidonic (AA) acids in phospholipids and cholesteryl esters, and increased concentrations of hydroxy-octadecadienoic acids (9-HODE and 13-HODE) and 15-hydroxy-eicosatetraenoic acid (15-HETE), derived from LA and AA respectively, especially hydroxy derivatives esterified in phospholipids. Glycoxidized HDL dose-dependently decreased collagen-induced platelet aggregation by binding to SR-BI. Glycoxidized HDL prevented collagen-induced increased phosphorylation of platelet p38 MAPK and cytosolic phospholipase A2, as well as intracellular calcium mobilization. HDL enriched with oxidized phospholipids, namely PC(16:0/13-HODE) dose-dependently inhibited platelet aggregation. Increased concentrations of 9-HODE, 13-HODE and 15-HETE in phospholipids (2.1, 2.1 and 2.4-fold increase respectively) were found in HDL from patients with T2D, and these HDL also inhibited platelet aggregation via SR-BI.CONCLUSIONS:Altogether, our results indicate that in vitro glycoxidized HDL as well as HDL from T2D patients inhibit platelet aggregation, and suggest that oxidized LA-containing phospholipids may contribute to the anti-aggregatory effects of glycoxidized HDL and HDL from T2D patients.
Type de document :
Article dans une revue
Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2015, pp.1-10. 〈10.1210/jc.2014-4214〉
Liste complète des métadonnées

Littérature citée [8 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01134619
Contributeur : Evelyne Vericel <>
Soumis le : mardi 24 mars 2015 - 08:40:53
Dernière modification le : mardi 17 juillet 2018 - 15:49:51
Document(s) archivé(s) le : mercredi 9 novembre 2016 - 12:12:00

Fichiers

JCEM Lê and al.pdf
Fichiers produits par l'(les) auteur(s)

  •  JCEM Lê et al., 2015.pdf Fichiers éditeurs autorisés sur une archive ouverte  - Commentaire : l'éditeur de "Endocrine Society" accepte la mise en ligne du fichier éditeur après un embargo d'1 an donc on l'a ajouté à ce dépôt.

Identifiants

Collections

Citation

Quang Huy Lê, Meddy El Alaoui, Evelyne Véricel, Bérénice Ségrestin, Laurent Soulère, et al.. Glycoxidized HDL, HDL enriched with oxidized phospholipids and HDL from diabetic patients inhibit platelet function. Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2015, pp.1-10. 〈10.1210/jc.2014-4214〉. 〈inserm-01134619〉

Partager

Métriques

Consultations de la notice

336

Téléchargements de fichiers

111