Toxoplasma gondii: Flat-mounting of retina as a new tool for the observation of ocular infection in mice

Abstract : Ocular toxoplasmosis is the principal cause of posterior uveitis and a leading cause of blindness. Animal models are required to improve our understanding of the pathogenesis of this disease. The method cur-rently used for the detection of retinal cysts in animals involves the observation, under a microscope, of all the sections from infected eyes. However, this method is time-consuming and lacks sensitivity. We have developed a rapid, sensitive method for observing retinal cysts in mice infected with Toxoplasma gondii. This method involves combining the flat-mounting of retina – a compromise between macro-scopic observation and global analysis of this tissue – and the use of an avirulent recombinant strain of T. gondii expressing the Escherichia coli b-galactosidase gene, visually detectable at the submacroscopic level. Single cyst unilateral infection was found in six out of 17 mice killed within 28 days of infection, whereas a bilateral infection was found in only one mouse. There was no correlation between brain cysts number and ocular infection.
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Experimental Parasitology, Elsevier, 2010, pp.259-262. 〈10.1016/j.exppara.2010.04.006〉
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Patricia Escoffier, Jean-Claude Jeanny, Carine Marinach-Patrice, Laurent Jonet, William Raoul, et al.. Toxoplasma gondii: Flat-mounting of retina as a new tool for the observation of ocular infection in mice. Experimental Parasitology, Elsevier, 2010, pp.259-262. 〈10.1016/j.exppara.2010.04.006〉. 〈inserm-01094666〉

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