A. R. Thierry, M. Florent, E. Safia, . Messaoudi, M. Caroline et al., Detection of KRAS and BRAF point mutations from circulating DNA analysis and concordance with tumor-tissue analysis in metastatic colorectal cancer, 2013.

R. G. Amado, M. Wolf, M. Peeters, E. Van-cutsem, S. Siena et al., Is Required for Panitumumab Efficacy in Patients With Metastatic Colorectal Cancer, Journal of Clinical Oncology, vol.26, issue.10, pp.1626-1660, 2008.
DOI : 10.1200/JCO.2007.14.7116

S. Bamford, E. Dawson, S. Forbes, J. Clements, R. Pettett et al., The COSMIC (Catalogue of Somatic Mutations in Cancer) database and website, British Journal of Cancer, vol.300, issue.912, pp.355-363, 2004.
DOI : 10.1093/nar/gkh073

S. A. Bustin, V. Benes, J. A. Garson, J. Hellemans, J. Huggett et al., The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments, Clinical Chemistry, vol.55, issue.4, pp.611-633, 2009.
DOI : 10.1373/clinchem.2008.112797

K. C. Chan, P. Jiang, Y. W. Zheng, G. J. Liao, H. Sun et al., Cancer Genome Scanning in Plasma: Detection of Tumor-Associated Copy Number Aberrations, Single-Nucleotide Variants, and Tumoral Heterogeneity by Massively Parallel Sequencing, Clinical Chemistry, vol.59, issue.1, pp.211-235, 2013.
DOI : 10.1373/clinchem.2012.196014

S. J. Dawson, D. W. Tsui, M. Murtaza, H. Biggs, O. M. Rueda et al., Analysis of Circulating Tumor DNA to Monitor Metastatic Breast Cancer, New England Journal of Medicine, vol.368, issue.13, pp.1199-209, 1056.
DOI : 10.1056/NEJMoa1213261

D. Nicolantonio, F. , S. Arena, M. Gallicchio, D. Zecchin et al., Replacement of normal with mutant alleles in the genome of normal human cells unveils mutationspecific drug responses, Proc Natl Acad Sci, issue.10552, pp.20864-20873, 2008.

D. Nicolantonio, F. , M. Martini, F. Molinari, A. Sartore-bianchi et al., Is Required for Response to Panitumumab or Cetuximab in Metastatic Colorectal Cancer, Journal of Clinical Oncology, vol.26, issue.35, pp.5705-5717, 2008.
DOI : 10.1200/JCO.2008.18.0786

F. Diehl, M. Li, D. Dressman, Y. He, D. Shen et al., Detection and quantification of mutations in the plasma of patients with colorectal tumors, Proceedings of the National Academy of Sciences, vol.102, issue.45, pp.16368-73, 2005.
DOI : 10.1073/pnas.0507904102

F. Diehl, K. Schmidt, M. A. Choti, K. Romans, S. Goodman et al., Circulating mutant DNA to assess tumor dynamics, Nature Medicine, vol.3, issue.9, pp.985-90, 2008.
DOI : 10.1038/nm.1789
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820391

F. Diehl, K. Schmidt, K. H. Durkee, K. J. Moore, S. N. Goodman et al., Analysis of Mutations in DNA Isolated From Plasma and Stool of Colorectal Cancer Patients, Gastroenterology, vol.135, issue.2, pp.489-98, 2008.
DOI : 10.1053/j.gastro.2008.05.039

J. Y. Douillard, K. S. Oliner, S. Siena, J. Tabernero, R. Burkes et al., Mutations in Colorectal Cancer, New England Journal of Medicine, vol.369, issue.11, pp.1023-1057, 1056.
DOI : 10.1056/NEJMoa1305275

E. Messaoudi, S. , F. Rolet, F. Mouliere, and A. R. Thierry, Circulating cell free DNA: Preanalytical considerations, Clinica Chimica Acta, vol.424, 2013.
DOI : 10.1016/j.cca.2013.05.022

J. Ellinger, V. Wittkamp, P. Albers, F. G. Perabo, S. C. Mueller et al., Cell-Free Circulating DNA: Diagnostic Value in Patients With Testicular Germ Cell Cancer, The Journal of Urology, vol.181, issue.1, pp.363-71, 2009.
DOI : 10.1016/j.juro.2008.08.118

M. Fleischhacker, B. Schmidt, S. Weickmann, D. M. Fersching, G. S. Leszinski et al., Methods for isolation of cell-free plasma DNA strongly affect DNA yield, Clinica Chimica Acta, vol.412, issue.23-24, pp.23-242085, 2011.
DOI : 10.1016/j.cca.2011.07.011

T. Forshew, M. Murtaza, C. Parkinson, D. Gale, D. W. Tsui et al., Noninvasive Identification and Monitoring of Cancer Mutations by Targeted Deep Sequencing of Plasma DNA, Science Translational Medicine, vol.4, issue.136, pp.136-68, 2012.
DOI : 10.1126/scitranslmed.3003726

D. C. García-olmo, C. Domínguez, M. García-arranz, P. Anker, M. Stroun et al., Cell-Free Nucleic Acids Circulating in the Plasma of Colorectal Cancer Patients Induce the Oncogenic Transformation of Susceptible Cultured Cells, Cancer Research, vol.70, issue.2, pp.560-567, 2010.
DOI : 10.1158/0008-5472.CAN-09-3513

T. M. Gorges, J. Schiller, A. Schmitz, D. Schuetzmann, C. Schatz et al., Cancer therapy monitoring in xenografts by quantitative analysis of circulating tumor DNA, Biomarkers, vol.25, issue.6, pp.498-506, 2012.
DOI : 10.1016/S0305-7372(02)00077-4

M. Guha, E. Castellanos-rizaldos, P. Liu, H. Mamon, and G. M. Makrigiorgos, Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations, Nucleic Acids Research, vol.41, issue.3, pp.50-60, 1250.
DOI : 10.1093/nar/gks1250

E. Heitzer, M. Auer, C. Gasch, M. Pichler, P. Ulz et al., Complex Tumor Genomes Inferred from Single Circulating Tumor Cells by Array-CGH and Next-Generation Sequencing, Cancer Research, vol.73, issue.10, pp.2965-75, 2013.
DOI : 10.1158/0008-5472.CAN-12-4140

C. Holohan, S. Van-schaeybroeck, D. B. Longley, and P. G. Johnston, Cancer drug resitance: an evolving paradigm, Nat Rev Cancer, pp.10-1038, 2013.
DOI : 10.1038/nrc3599

K. Jung, M. Fleischhacker, and A. Rabien, Cell-free DNA in the blood as a solid tumor biomarker???A critical appraisal of the literature, Clinica Chimica Acta, vol.411, issue.21-22, pp.21-221611, 2010.
DOI : 10.1016/j.cca.2010.07.032

S. A. Leon, B. Shapiro, D. M. Sklaroff, and M. J. Yaros, Free DNA in the serum of cancer patients and the effect of therapy, Cancer Res, issue.373, pp.646-50, 1977.

A. Lièvre, J. B. Bachet, V. Boige, A. Cayre, D. Le-corre et al., Mutations As an Independent Prognostic Factor in Patients With Advanced Colorectal Cancer Treated With Cetuximab, Journal of Clinical Oncology, vol.26, issue.3, pp.374-383, 2008.
DOI : 10.1200/JCO.2007.12.5906

A. Lièvre, J. B. Bachet, D. Le-corre, V. Boige, B. Landi et al., KRAS Mutation Status Is Predictive of Response to Cetuximab Therapy in Colorectal Cancer, Cancer Research, vol.66, issue.8, pp.3992-3997, 2006.
DOI : 10.1158/0008-5472.CAN-06-0191

S. Misale, R. Yaeger, S. Hobor, E. Scala, M. Janakiraman et al., Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer, Nature, vol.102, issue.4867404, pp.532-538, 2012.
DOI : 10.1038/nature11156

F. Mouliere, S. Messaoudi, C. Gongora, A. S. Guedj, B. Robert et al., Circulating Cell-Free DNA from Colorectal Cancer Patients May Reveal High KRAS or BRAF Mutation Load, Translational Oncology, vol.6, issue.3, pp.319-347, 2013.
DOI : 10.1593/tlo.12445
URL : https://hal.archives-ouvertes.fr/hal-00872683

F. Mouliere, E. A. Robert, M. Peyrotte, M. Del-rio, F. Ychou et al., High Fragmentation Characterizes Tumour-Derived Circulating DNA, PLoS ONE, vol.27, issue.9, 2011.
DOI : 10.1371/journal.pone.0023418.s003
URL : http://doi.org/10.1371/journal.pone.0023418

F. Mouliere and A. R. Thierry, The importance of examining the proportion of circulating DNA originating from tumor, microenvironment and normal cells in colorectal cancer patients, Expert Opinion on Biological Therapy, vol.25, issue.3, pp.209-224, 2012.
DOI : 10.1111/j.1365-2567.2005.02130.x

M. Murtaza, S. J. Dawson, D. W. Tsui, D. Gale, T. Forshew et al., Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA, Nature, vol.405, issue.7447, pp.108-120, 1038.
DOI : 10.1038/nature12065

A. Narayan, N. J. Carriero, S. N. Gettinger, J. Kluytenaar, K. R. Kozak et al., Ultrasensitive Measurement of Hotspot Mutations in Tumor DNA in Blood Using Error-Suppressed Multiplexed Deep Sequencing, Cancer Research, vol.72, issue.14, pp.3492-3500, 2012.
DOI : 10.1158/0008-5472.CAN-11-4037

D. Pang, T. A. Winters, M. Jung, S. Purkayastha, L. R. Cavalli et al., Radiation-generated Short DNA Fragments May Perturb Non-homologous End-joining and Induce Genomic Instability, Journal of Radiation Research, vol.52, issue.3, pp.309-328, 2011.
DOI : 10.1269/jrr.10147

P. Artru, M. Ducreux, A. Lièvre, M. Guiu, J. Merlin et al., Review of the current status of KRAS mutation testing in France in 2011: The Flash-KRAS study, Journal of Clinical Oncology, vol.14129, issue.30, 2012.
URL : https://hal.archives-ouvertes.fr/hal-00747407

J. S. Banerji and . De-bono, Multi-purpose utility of circulating plasma DNA testing in patients with advanced cancers, PLoS One, issue.711, 2012.

E. Y. Rykova, E. S. Morozkin, A. A. Ponomaryova, E. M. Loseva, I. A. Zaporozhchenko et al., Cell-free and cell-bound circulating nucleic acid complexes: mechanisms of generation, concentration and content, Expert Opinion on Biological Therapy, vol.28, issue.4, pp.141-53, 2012.
DOI : 10.1111/j.1464-410X.2006.06352.x

F. Salvianti, P. Pinzani, P. Verderio, C. M. Ciniselli, D. Massi et al., Multiparametric Analysis of Cell-Free DNA in Melanoma Patients, PLoS ONE, vol.5, issue.11, 2012.
DOI : 10.1371/journal.pone.0049843.s002

B. Schmidt, S. Weickmann, C. Witt, and M. Fleischhacker, Integrity of Cell-Free Plasma DNA in Patients with Lung Cancer and Nonmalignant Lung Disease, Annals of the New York Academy of Sciences, vol.77, issue.1, 2008.
DOI : 10.1196/annals.1448.034

S. Schwartz, E. Meshorer, and G. Ast, Chromatin organization marks exon-intron structure, Nature Structural & Molecular Biology, vol.448, issue.9, 2009.
DOI : 10.1038/nsmb.1659

H. Schwarzenbach, D. S. Hoon, and K. Pantel, Cell-free nucleic acids as biomarkers in cancer patients, Nature Reviews Cancer, vol.61, issue.83, pp.426-463, 2011.
DOI : 10.1038/nrc3066

K. L. Spindler, N. Pallisgaard, I. Vogelius, and A. Jakobsen, Quantitative Cell-Free DNA, KRAS, and BRAF Mutations in Plasma from Patients with Metastatic Colorectal Cancer during Treatment with Cetuximab and Irinotecan, Clinical Cancer Research, vol.18, issue.4, pp.1177-85, 2012.
DOI : 10.1158/1078-0432.CCR-11-0564

M. Stroun, P. Anker, J. Lyautey, C. Lederrey, and P. A. Maurice, Isolation and characterization of DNA from the plasma of cancer patients, European Journal of Cancer and Clinical Oncology, vol.23, issue.6, pp.707-719, 1987.
DOI : 10.1016/0277-5379(87)90266-5

M. Stroun, J. Lyautey, C. Lederrey, A. Olson-sand, and P. Anker, About the possible origin and mechanism of circulating DNA, Clinica Chimica Acta, vol.313, issue.1-2, pp.139-181, 2001.
DOI : 10.1016/S0009-8981(01)00665-9

V. Taly, D. Pekin, A. Abed, and P. Laurent-puig, Detecting biomarkers with microdroplet technology, Trends in Molecular Medicine, vol.18, issue.7, pp.405-421, 2012.
DOI : 10.1016/j.molmed.2012.05.001

A. R. Thierry, F. Mouliere, C. Gongora, J. Ollier, B. Robert et al., Origin and quantification of circulating DNA in mice with human colorectal cancer xenografts, Nucleic Acids Research, vol.38, issue.18, pp.6159-6175, 2010.
DOI : 10.1093/nar/gkq421

E. Van-cutsem, C. H. Köhne, I. Láng, G. Folprecht, M. P. Nowacki et al., Mutation Status, Journal of Clinical Oncology, vol.29, issue.15, pp.2011-2020, 2011.
DOI : 10.1200/JCO.2010.33.5091

M. Van-der-vaart and P. J. Pretorius, Is the role of circulating DNA as a biomarker of cancer being prematurely overrated?, Clinical Biochemistry, vol.43, issue.1-2, pp.26-36, 2010.
DOI : 10.1016/j.clinbiochem.2009.08.027

N. P. West, M. Dattani, P. Mcshane, G. Hutchins, J. Grabsch et al., The proportion of tumour cells is an independent predictor for survival in colorectal cancer patients, British Journal of Cancer, vol.26, issue.10, pp.1519-1542, 2010.
DOI : 10.1016/j.humpath.2007.04.008

A. S. Whale, J. F. Huggett, S. Cowen, V. Speirs, J. Shaw et al., Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation, Nucleic Acids Research, vol.40, issue.11, 2012.
DOI : 10.1093/nar/gks203