13 C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes.

Abstract : : BackgroundIt has been suggested that the adipokine resistin links obesity and insulin resistance, although how resistin acts on muscle metabolism is controversial. We aimed to quantitatively analyse the effects of resistin on the glucose metabolic flux profile and on insulin response in L6E9 myotubes at the metabolic level using a tracer-based metabolomic approach and our in-house developed software, Isodyn.ResultsResistin significantly increased glucose uptake and glycolysis, altering pyruvate utilisation by the cell. In the presence of resistin, insulin only slightly increased glucose uptake and glycolysis, and did not alter the flux profile around pyruvate induced by resistin. Resistin prevented the increase in gene expression in pyruvate dehydrogenase-E1 and the sharp decrease in gene expression in cytosolic phosphoenolpyruvate carboxykinase-1 induced by insulin.ConclusionsThese data suggest that resistin impairs the metabolic activation of insulin. This impairment cannot be explained by the activity of a single enzyme, but instead due to reorganisation of the whole metabolic flux distribution.
Type de document :
Article dans une revue
BMC Systems Biology, BioMed Central, 2014, 8 (1), pp.109. 〈10.1186/s12918-014-0109-z〉
Liste complète des métadonnées

Littérature citée [48 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01073652
Contributeur : Christine Dupuis <>
Soumis le : vendredi 10 octobre 2014 - 11:26:56
Dernière modification le : vendredi 28 novembre 2014 - 01:00:52

Annexe

Identifiants

Collections

Citation

Shirley Guzmán, Silvia Marin, Anibal Miranda, Vitaly Selivanov, Josep Centelles, et al.. 13 C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes.. BMC Systems Biology, BioMed Central, 2014, 8 (1), pp.109. 〈10.1186/s12918-014-0109-z〉. 〈inserm-01073652〉

Partager

Métriques

Consultations de la notice

138

Téléchargements de fichiers

13