IGF2 promotes growth of adrenocortical carcinoma cells, but its overexpression does not modify phenotypic and molecular features of adrenocortical carcinoma.

Abstract : Insulin-like growth factor 2 (IGF2) overexpression is an important molecular marker of adrenocortical carcinoma (ACC), which is a rare but devastating endocrine cancer. It is not clear whether IGF2 overexpression modifies the biology and growth of this cancer, thus more studies are required before IGF2 can be considered as a major therapeutic target. We compared the phenotypical, clinical, biological, and molecular characteristics of ACC with or without the overexpression of IGF2, to address these issues. We also carried out a similar analysis in an ACC cell line (H295R) in which IGF2 expression was knocked down with si- or shRNA. We found no significant differences in the clinical, biological and molecular (transcriptomic) traits between IGF2-high and IGF2-low ACC. The absence of IGF2 overexpression had little influence on the activation of tyrosine kinase pathways both in tumors and in H295 cells that express low levels of IGF2. In IGF2-low tumors, other growth factors (FGF9, PDGFA) are more expressed than in IGF2-high tumors, suggesting that they play a compensatory role in tumor progression. In addition, IGF2 knock-down in H295R cells substantially impaired growth (>50% inhibition), blocked cells in G1 phase, and promoted apoptosis (>2-fold). Finally, analysis of the 11p15 locus showed a paternal uniparental disomy in both IGF2-high and IGF2-low tumors, but low IGF2 expression could be explained in most IGF2-low ACC by an additional epigenetic modification at the 11p15 locus. Altogether, these observations confirm the active role of IGF2 in adrenocortical tumor growth, but also suggest that other growth promoting pathways may be involved in a subset of ACC with low IGF2 expression, which creates opportunities for the use of other targeted therapies.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2014, 9 (8), pp.e103744. 〈10.1371/journal.pone.0103744〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-01068039
Contributeur : Mireille Bos <>
Soumis le : mercredi 24 septembre 2014 - 16:12:24
Dernière modification le : mercredi 21 mars 2018 - 18:57:57
Document(s) archivé(s) le : jeudi 25 décembre 2014 - 11:05:11

Fichier

journal.pone.0103744.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Marine Guillaud-Bataille, Bruno Ragazzon, Aurélien De Reyniès, Claire Chevalier, Isabelle Francillard, et al.. IGF2 promotes growth of adrenocortical carcinoma cells, but its overexpression does not modify phenotypic and molecular features of adrenocortical carcinoma.. PLoS ONE, Public Library of Science, 2014, 9 (8), pp.e103744. 〈10.1371/journal.pone.0103744〉. 〈inserm-01068039〉

Partager

Métriques

Consultations de la notice

497

Téléchargements de fichiers

238