Analysis of perfusates during hypothermic machine perfusion by NMR spectroscopy: a potential tool for predicting kidney graft outcome.

Delphine Bon 1 Claire Billault 2 Raphaël Thuillier 1, 3, 4 William Hebrard 5 Nadège Boildieu 1 Olivier Celhay 6 Jacques Irani 6 François Seguin 1 Thierry Hauet 1
1 IRTOMIT - Ischémie Reperfusion en Transplantation d’Organes Mécanismes et Innovations Thérapeutiques
2 Service d'urologie et transplantation rénales [CHU Pitié-Salpétrière]
3 Consortium for Organ Preservation in Europe
4 Service de biochimie
5 Ibisa - Groupement Scientifique - Ibisa
6 Service d'Urologie
Abstract : BACKGROUND: Machine perfusion use has been reported to promote graft outcome in case of donation after cardiac death. Our objective was to evaluate the potential for nuclear magnetic resonance (NMR) to predict graft outcome by analyzing perfusates during machine perfusion time. METHOD: We used a renal autotransplantation model mimicking deceased after cardiac death donors with pigs. Organs were subjected to 60 min of warm ischemia before the hypothermic machine preservation during 22 hr. We studied the correlation between creatinemia after transplantation and the NMR data from perfusates. RESULTS: A metabonomic analysis allowed us to highlight the evolution of several metabolites during perfusion: the concentration of lactate, choline, or amino acids such as valine, glycine, or glutamate increased with time, whereas there was a diminution of total glutathione during this period. The changes in these biomarkers were less severe in the group with the better outcome. Statistical analysis revealed a strong association between the level of those metabolites during machine perfusion and function recovery (Spearman rank ≥0.89; P<0.05). CONCLUSION: Multivariate analysis of lesion biomarkers during kidney perfusion using NMR data could be an interesting tool to assess graft quality, particularly because analyses times (2 hr total) are compatible with clinical application.
Type de document :
Article dans une revue
Transplantation, Lippincott, Williams & Wilkins, 2014, 97 (8), pp.810-6. 〈10.1097/TP.0000000000000046〉
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http://www.hal.inserm.fr/inserm-01057477
Contributeur : Delphine Bon <>
Soumis le : vendredi 22 août 2014 - 15:51:25
Dernière modification le : lundi 8 janvier 2018 - 17:48:01

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UNIV-POITIERS | INRA | IRTOMIT

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Delphine Bon, Claire Billault, Raphaël Thuillier, William Hebrard, Nadège Boildieu, et al.. Analysis of perfusates during hypothermic machine perfusion by NMR spectroscopy: a potential tool for predicting kidney graft outcome.. Transplantation, Lippincott, Williams & Wilkins, 2014, 97 (8), pp.810-6. 〈10.1097/TP.0000000000000046〉. 〈inserm-01057477〉

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