Myotubularin and PtdIns3P remodel the sarcoplasmic reticulum in muscle in vivo.

Abstract : The sarcoplasmic reticulum (SR) is a specialized form of endoplasmic reticulum (ER) in skeletal muscle and is essential for calcium homeostasis. The mechanisms involved in SR remodeling and maintenance of SR subdomains are elusive. In this study, we identified myotubularin (MTM1), a phosphoinositide phosphatase mutated in X-linked centronuclear myopathy (XLCNM, or myotubular myopathy), as a key regulator of phosphatidylinositol 3-monophosphate (PtdIns3P) levels at the SR. MTM1 is predominantly located at the SR cisternae of the muscle triads, and Mtm1-deficient mouse muscles and myoblasts from XLCNM patients exhibit abnormal SR/ER networks. In vivo modulation of MTM1 enzymatic activity in skeletal muscle using ectopic expression of wild-type or a dead-phosphatase MTM1 protein leads to differential SR remodeling. Active MTM1 is associated with flat membrane stacks, whereas dead-phosphatase MTM1 mutant promotes highly curved cubic membranes originating from the SR and enriched in PtdIns3P. Overexpression of a tandem FYVE domain with high affinity for PtdIns3P alters the shape of the SR cisternae at the triad. Our findings, supported by the parallel analysis of the Mtm1-null mouse and an in vivo study, reveal a direct function of MTM1 enzymatic activity in SR remodeling and a key role for PtdIns3P in promoting SR membrane curvature in skeletal muscle. We propose that alteration in SR remodeling is a primary cause of X-linked centronuclear myopathy. The tight regulation of PtdIns3P on specific membrane subdomains may be a general mechanism to control membrane curvature.
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Journal of Cell Science, Company of Biologists, 2013, 126 (Pt 8), pp.1806-19. 〈10.1242/jcs.118505〉
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Soumis le : mercredi 25 juin 2014 - 13:07:20
Dernière modification le : vendredi 1 juin 2018 - 10:52:04

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Leonela Amoasii, Karim Hnia, Gaëtan Chicanne, Andreas Brech, Belinda S Cowling, et al.. Myotubularin and PtdIns3P remodel the sarcoplasmic reticulum in muscle in vivo.. Journal of Cell Science, Company of Biologists, 2013, 126 (Pt 8), pp.1806-19. 〈10.1242/jcs.118505〉. 〈inserm-01012056〉

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