Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon

Abstract : Background
The identification of novel giant viruses from the nucleocytoplasmic large DNA viruses group and their virophages has increased in the last decade and has helped to shed light on viral evolution. This study describe the discovery, isolation and characterization of Samba virus (SMBV), a novel giant virus belonging to the Mimivirus genus, which was isolated from the Negro River in the Brazilian Amazon. We also report the isolation of an SMBV-associated virophage named Rio Negro (RNV), which is the first Mimivirus virophage to be isolated in the Americas.
Methods/results
Based on a phylogenetic analysis, SMBV belongs to group A of the putative Megavirales order, possibly a new virus related to Acanthamoeba polyphaga mimivirus (APMV). SMBV is the largest virus isolated in Brazil, with an average particle diameter about 574 nm. The SMBV genome contains 938 ORFs, of which nine are ORFans. The 1,213.6 kb SMBV genome is one of the largest genome of any group A Mimivirus described to date. Electron microscopy showed RNV particle accumulation near SMBV and APMV factories resulting in the production of defective SMBV and APMV particles and decreasing the infectivity of these two viruses by several logs.
Conclusion
This discovery expands our knowledge of Mimiviridae evolution and ecology.
Type de document :
Article dans une revue
Virology Journal, BioMed Central, 2014, 11 (1), pp.95
Liste complète des métadonnées

Littérature citée [29 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00990732
Contributeur : Ed. Bmc <>
Soumis le : mercredi 14 mai 2014 - 09:07:02
Dernière modification le : lundi 12 mars 2018 - 13:48:01
Document(s) archivé(s) le : jeudi 14 août 2014 - 11:05:54

Identifiants

  • HAL Id : inserm-00990732, version 1

Collections

Citation

Rafael Campos, Paulo Boratto, Felipe Assis, Eric Aguiar, Lorena Silva, et al.. Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon. Virology Journal, BioMed Central, 2014, 11 (1), pp.95. 〈inserm-00990732〉

Partager

Métriques

Consultations de la notice

208

Téléchargements de fichiers

248