Survival of taylorellae in the environmental amoeba Acanthamoeba castellanii

Abstract : Background
Taylorella equigenitalis is the causative agent of contagious equine metritis, a sexually-transmitted infection of Equidae characterised in infected mares by abundant mucopurulent vaginal discharge and a variable degree of vaginitis, cervicitis or endometritis, usually resulting in temporary infertility. The second species of the Taylorella genus, Taylorella asinigenitalis, is considered non-pathogenic, although mares experimentally infected with this bacterium can develop clinical signs of endometritis. To date, little is understood about the basic molecular virulence and persistence mechanisms employed by the Taylorella species. To clarify these points, we investigated whether the host-pathogen interaction model Acanthamoeba castellanii was a suitable model for studying taylorellae.
Results
We herein demonstrate that both species of the Taylorella genus are internalised by a mechanism involving the phagocytic capacity of the amoeba and are able to survive for at least one week inside the amoeba. During this one-week incubation period, taylorellae concentrations remain strikingly constant and no overt toxicity to amoeba cells was observed.
Conclusions
This study provides the first evidence of the capacity of taylorellae to survive in a natural environment other than the mammalian genital tract, and shows that the alternative infection model, A. castellanii, constitutes a relevant alternative system to assess host-pathogen interactions of taylorellae. The survival of taylorellae inside the potential environmental reservoir A. castellanii brings new insight, fostering a broader understanding of taylorellae biology and its potential natural ecological niche.
Type de document :
Article dans une revue
BMC Microbiology, BioMed Central, 2014, 14 (1), pp.69. 〈10.1186/1471-2180-14-69〉
Liste complète des métadonnées

Littérature citée [33 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00980498
Contributeur : Ed. Bmc <>
Soumis le : vendredi 18 avril 2014 - 10:19:20
Dernière modification le : jeudi 15 mars 2018 - 10:13:15
Document(s) archivé(s) le : lundi 10 avril 2017 - 15:30:19

Fichiers

Identifiants

Collections

Citation

Julie Allombert, Anne Vianney, Claire Laugier, Sandrine Petry, Laurent Hébert. Survival of taylorellae in the environmental amoeba Acanthamoeba castellanii. BMC Microbiology, BioMed Central, 2014, 14 (1), pp.69. 〈10.1186/1471-2180-14-69〉. 〈inserm-00980498〉

Partager

Métriques

Consultations de la notice

706

Téléchargements de fichiers

188