Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia.

Abstract : BACKGROUND: TiO2 particles are commonly used as dietary supplements and may contain up to 36% of nano-sized particles (TiO2-NPs). Still impact and translocation of NPs through the gut epithelium is poorly documented. RESULTS: We show that, in vivo and ex vivo, agglomerates of TiO2-NPs cross both the regular ileum epithelium and the follicle-associated epithelium (FAE) and alter the paracellular permeability of the ileum and colon epithelia. In vitro, they accumulate in M-cells and mucus-secreting cells, much less in enterocytes. They do not cause overt cytotoxicity or apoptosis. They translocate through a model of FAE only, but induce tight junctions remodeling in the regular ileum epithelium, which is a sign of integrity alteration and suggests paracellular passage of NPs. Finally we prove that TiO2-NPs do not dissolve when sequestered up to 24 h in gut cells. CONCLUSIONS: Taken together these data prove that TiO2-NPs would possibly translocate through both the regular epithelium lining the ileum and through Peyer's patches, would induce epithelium impairment, and would persist in gut cells where they would possibly induce chronic damage.
Type de document :
Article dans une revue
Particle and Fibre Toxicology, BioMed Central, 2014, 11 (1), pp.13. 〈10.1186/1743-8977-11-13〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00971511
Contributeur : Ed. Bmc <>
Soumis le : jeudi 3 avril 2014 - 01:20:00
Dernière modification le : jeudi 28 juin 2018 - 09:51:49
Document(s) archivé(s) le : jeudi 3 juillet 2014 - 11:01:00

Identifiants

Citation

Emilie Brun, Frédérick Barreau, Giulia Veronesi, Barbara Fayard, Stéphanie Sorieul, et al.. Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia.. Particle and Fibre Toxicology, BioMed Central, 2014, 11 (1), pp.13. 〈10.1186/1743-8977-11-13〉. 〈inserm-00971511〉

Partager

Métriques

Consultations de la notice

917

Téléchargements de fichiers

1608