Neuronal transport defects of the MAP6 KO mouse - a model of schizophrenia - and alleviation by Epothilone D treatment, as observed using MEMRI.

Alexia Daoust 1 Sylvain Bohic 2 Yasmina Saoudi 3 Clément Stéphan Debacker 4 Sylvie Gory-Fauré 5 Annie Andrieux 5 Emmanuel Barbier 6, * Jean-Christophe Deloulme 5, *
* Auteur correspondant
2 INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale
ESRF - European Synchrotron Radiation Facility, GIN - Grenoble Institut des Neurosciences
3 INSERM U836, équipe 1, Physiopathologie du cytosquelette
ESRF - European Synchrotron Radiation Facility, GIN - Grenoble Institut des Neurosciences, Commissariat à l'Energie Atomique et aux Energies Alternatives
4 INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
GIN - Grenoble Institut des Neurosciences, Bruker Biospin MRI
5 INSERM U836, équipe 1, Physiopathologie du cytosquelette
GIN - Grenoble Institut des Neurosciences, Commissariat à l'Energie Atomique et aux Energies Alternatives
6 INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
ESRF - European Synchrotron Radiation Facility, GIN - Grenoble Institut des Neurosciences
Abstract : The MAP6 (microtubule-associated protein 6) KO mouse is a microtubule-deficient model of schizophrenia that exhibits severe behavioral disorders that are associated with synaptic plasticity anomalies. These defects are alleviated not only by neuroleptics, which are the gold standard molecules for the treatment of schizophrenia, but also by Epothilone D (Epo D), which is a microtubule-stabilizing molecule. To compare the neuronal transport between MAP6 KO and wild-type mice and to measure the effect of Epo D treatment on neuronal transport in KO mice, MnCl2 was injected in the primary somatosensory cortex. Then, using manganese-enhanced magnetic resonance imaging (MEMRI), we followed the propagation of Mn(2+) through axonal tracts and brain regions that are connected to the somatosensory cortex. In MAP6 KO mice, the measure of the MRI relative signal intensity over 24h revealed that the Mn(2+) transport rate was affected with a stronger effect on long-range and polysynaptic connections than in short-range and monosynaptic tracts. The chronic treatment of MAP6 KO mice with Epo D strongly increased Mn(2+) propagation within both mono- and polysynaptic connections. Our results clearly indicate an in vivo deficit in neuronal Mn(2+) transport in KO MAP6 mice, which might be due to both axonal transport defects and synaptic transmission impairments. Epo D treatment alleviated the axonal transport defects, and this improvement most likely contributes to the positive effect of Epo D on behavioral defects in KO MAP6 mice.
Type de document :
Article dans une revue
NeuroImage, Elsevier, 2014, 96, pp.133-142. 〈10.1016/j.neuroimage.2014.03.071〉
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http://www.hal.inserm.fr/inserm-00968900
Contributeur : Annie Andrieux <>
Soumis le : vendredi 25 avril 2014 - 13:36:16
Dernière modification le : jeudi 1 février 2018 - 01:11:50

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Alexia Daoust, Sylvain Bohic, Yasmina Saoudi, Clément Stéphan Debacker, Sylvie Gory-Fauré, et al.. Neuronal transport defects of the MAP6 KO mouse - a model of schizophrenia - and alleviation by Epothilone D treatment, as observed using MEMRI.. NeuroImage, Elsevier, 2014, 96, pp.133-142. 〈10.1016/j.neuroimage.2014.03.071〉. 〈inserm-00968900〉

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