PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.

Abstract : The spindle checkpoint delays anaphase onset until every chromosome kinetochore has been efficiently captured by the mitotic spindle microtubules. In this study, we report that the human pre-messenger RNA processing 4 (PRP4) protein kinase associates with kinetochores during mitosis. PRP4 depletion by RNA interference induces mitotic acceleration. Moreover, we frequently observe lagging chromatids during anaphase leading to aneuploidy. PRP4-depleted cells do not arrest in mitosis after nocodazole treatment, indicating a spindle assembly checkpoint (SAC) failure. Thus, we find that PRP4 is necessary for recruitment or maintenance of the checkpoint proteins MPS1, MAD1, and MAD2 at the kinetochores. Our data clearly identify PRP4 as a previously unrecognized kinetochore component that is necessary to establish a functional SAC.
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Journal of Cell Biology, Rockefeller University Press, 2007, 179 (4), pp.601-9. 〈10.1083/jcb.200703133〉
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Contributeur : Claude Prigent <>
Soumis le : jeudi 27 mars 2014 - 09:24:06
Dernière modification le : mercredi 16 mai 2018 - 11:23:33

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Emilie Montembault, Stéphanie Dutertre, Claude Prigent, Régis Giet. PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.. Journal of Cell Biology, Rockefeller University Press, 2007, 179 (4), pp.601-9. 〈10.1083/jcb.200703133〉. 〈inserm-00966663〉

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