The Xenopus laevis centrosome aurora/Ipl1-related kinase.

Abstract : The cDNA encoding the protein kinase pEg2 was originally cloned through a differential screening performed during the early development of Xenopus laevis. pEg2 orthologues were found in various organisms and were classified in a new family of oncogenic mitotic protein kinases named 'aurora/Ipl1-related kinases' after the Drosophila melanogaster gene aurora and the Saccharomyces cerevisiae gene Ipl1. The catalytic activity of pEg2 is necessary for the mitotic microtubule spindle formation in Xenopus laevis egg extracts. The addition of a dominant negative form of pEg2 to in vitro spindle assembly assays leads to monopolar spindles generated by a defect of centrosome separation. In Xenopus cultured cells, pEg2 was confined around the pericentriolar material once centrosomes were duplicated. The centrosome localization does not depend on the presence of microtubules. However, in vitro, the protein binds to taxol-stabilized microtubules independently of its kinase activity. During mitosis the location of the protein changes, in metaphase the kinase localizes on the microtubules at the poles of the mitotic spindle whereas it is not present on astral microtubules. This localization persists until the segregation of the chromosomes is completed. The presence of the kinase on the spindle may reveal another yet unknown function.
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Article dans une revue
Biology of the Cell, Wiley, 1999, 91 (6), pp.461-70
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http://www.hal.inserm.fr/inserm-00966131
Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 11:00:30
Dernière modification le : mercredi 16 mai 2018 - 11:24:08

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  • HAL Id : inserm-00966131, version 1
  • PUBMED : 10519006

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Régis Giet, Rustem Uzbekov, Igor Kireev, Claude Prigent. The Xenopus laevis centrosome aurora/Ipl1-related kinase.. Biology of the Cell, Wiley, 1999, 91 (6), pp.461-70. 〈inserm-00966131〉

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