Reinvestigation of DNA ligase I in axolotl and Pleurodeles development.

Abstract : We have recently shown that the exclusion process causing the replacement of DNA ligases II by DNA ligase I in amphibian eggs after fertilization does not occur in the case of Xenopus laevis [Hardy, S., Aoufouchi, S., Thiebaud, P., and Prigent, C., (1991) Nucleic Acids Res. 19, 701-705]. Since this result is in contradiction with the situation reported in axolotl and Pleurodeles we decided to reinvestigate such results in both species. Three different approaches have been used: (1) the substrate specificity of DNA ligase I; (2) the DNA ligase-AMP adduct reaction and (3) the immunological detection using antibodies raised against the X.laevis DNA ligase I. Our results clearly demonstrate that DNA ligase I activity is associated with a single polypeptide which is present in oocyte, unfertilized egg and embryo of both amphibians. Therefore, the hypothesis of a change in DNA ligase forms, resulting from an expression of the DNA ligase I gene in axolotl and Pleurodeles early development must be rejected. We also show that, in contradiction with published data, the unfertilized sea urchin egg contains a DNA ligase activity able to join blunt ended DNA molecules.
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Article dans une revue
Nucleic Acids Research, Oxford University Press, 1991, 19 (16), pp.4395-8
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Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 08:13:06
Dernière modification le : mercredi 16 mai 2018 - 11:24:08

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  • HAL Id : inserm-00966021, version 1
  • PUBMED : 1886765

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Saïd Aoufouchi, Serge Hardy, Claude Prigent, Michel Philippe, Pierre Thiebaud. Reinvestigation of DNA ligase I in axolotl and Pleurodeles development.. Nucleic Acids Research, Oxford University Press, 1991, 19 (16), pp.4395-8. 〈inserm-00966021〉

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