Combining Ca2+ and membrane potential imaging in single neurons. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Cold Spring Harbor protocols Année : 2013

Combining Ca2+ and membrane potential imaging in single neurons.

Résumé

The ability to monitor Ca(2+) signals and membrane potential simultaneously at multiple locations on the same neuron facilitates further progress in our understanding of neuronal function. In particular, this method allows correlation of electrical and chemical signals from multiple sites, including those inaccessible to microelectrodes. This protocol describes a procedure for loading cells with two indicators, a Ca(2+)-sensitive Fura dye and voltage-sensitive JPW1114, together with the equipment required for detecting and imaging the two signals. Potential problems are discussed as well as the capabilities and limitations of the technique.
Fichier principal
Vignette du fichier
Canepari-2013_Combining_Ca2_Protocole_1-AA.pdf (170.65 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

inserm-00951450 , version 1 (24-02-2014)

Identifiants

Citer

Marco Canepari, Kaspar E. Vogt, Michel de Waard, Dejan Zecevic. Combining Ca2+ and membrane potential imaging in single neurons.. Cold Spring Harbor protocols, 2013, 2013 (12), pp.1161-4. ⟨10.1101/pdb.prot073114⟩. ⟨inserm-00951450⟩

Collections

INSERM UGA U836
155 Consultations
300 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More