Combining Ca2+ and membrane potential imaging in single neurons.

Marco Canepari; Kaspar Vogt; Michel De Waard; Dejan Zecevic

doi:10.1101/pdb.prot073114

Combining Ca2+ and membrane potential imaging in single neurons.

Marco Canepari ^{1, *} Kaspar Vogt ² Michel De Waard ¹ Dejan Zecevic ³

* Auteur correspondant

1 INSERM U836, équipe 3, Canaux calciques, fonctions et pathologies

GIN - Grenoble Institut des Neurosciences

2 Department of Pharmacology and Neurobiology

3 Department of Cellular and Molecular Physiology

Abstract : The ability to monitor Ca(2+) signals and membrane potential simultaneously at multiple locations on the same neuron facilitates further progress in our understanding of neuronal function. In particular, this method allows correlation of electrical and chemical signals from multiple sites, including those inaccessible to microelectrodes. This protocol describes a procedure for loading cells with two indicators, a Ca(2+)-sensitive Fura dye and voltage-sensitive JPW1114, together with the equipment required for detecting and imaging the two signals. Potential problems are discussed as well as the capabilities and limitations of the technique.

Type de document :

Article dans une revue

Cold Spring Harbor protocols, Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, 2013, 2013 (12), pp.1161-4. 〈10.1101/pdb.prot073114〉

Domaine :

Sciences du Vivant [q-bio] / Neurosciences [q-bio.NC]

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http://www.hal.inserm.fr/inserm-00951450
Contributeur : Marco Canepari <>
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Dernière modification le : jeudi 1 février 2018 - 01:11:56
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