IL-17A/F-signaling does not contribute to the initial phase of mucosal inflammation triggered by S. Typhimurium.

Abstract : Salmonella enterica subspecies 1 serovar Typhimurium (S. Typhimurium) causes diarrhea and acute inflammation of the intestinal mucosa. The pro-inflammatory cytokines IL-17A and IL-17F are strongly induced in the infected mucosa but their contribution in driving the tissue inflammation is not understood. We have used the streptomycin mouse model to analyze the role of IL-17A and IL-17F and their cognate receptor IL-17RA in S. Typhimurium enterocolitis. Neutralization of IL-17A and IL-17F did not affect mucosal inflammation triggered by infection or spread of S. Typhimurium to systemic sites by 48 h p.i. Similarly, Il17ra(-/-) mice did not display any reduction in infection or inflammation by 12 h p.i. The same results were obtained using S. Typhimurium variants infecting via the TTSS1 type III secretion system, the TTSS1 effector SipA or the TTSS1 effector SopE. Moreover, the expression pattern of 45 genes encoding chemokines/cytokines (including CXCL1, CXCL2, IL-17A, IL-17F, IL-1α, IL-1β, IFNγ, CXCL-10, CXCL-9, IL-6, CCL3, CCL4) and antibacterial molecules was not affected by Il17ra deficiency by 12 h p.i. Thus, in spite of the strong increase in Il17a/Il17f mRNA in the infected mucosa, IL-17RA signaling seems to be dispensable for eliciting the acute disease. Future work will have to address whether this is attributable to redundancy in the cytokine signaling network.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2010, 5 (11), pp.e13804. 〈10.1371/journal.pone.0013804〉
Liste complète des métadonnées

Littérature citée [37 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00926613
Contributeur : Jean-Claude Sirard <>
Soumis le : vendredi 10 janvier 2014 - 13:21:53
Dernière modification le : mardi 3 juillet 2018 - 11:45:38
Document(s) archivé(s) le : jeudi 10 avril 2014 - 22:06:41

Fichier

journal.pone.0013804.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Pascal Songhet, Manja Barthel, Till A Röhn, Laurye Van Maele, Delphine Cayet, et al.. IL-17A/F-signaling does not contribute to the initial phase of mucosal inflammation triggered by S. Typhimurium.. PLoS ONE, Public Library of Science, 2010, 5 (11), pp.e13804. 〈10.1371/journal.pone.0013804〉. 〈inserm-00926613〉

Partager

Métriques

Consultations de la notice

472

Téléchargements de fichiers

129