Determination of dodine in fruit samples by liquid chromatography electrospray ionization-tandem mass spectrometry.

Abstract : A rapid and sensitive LC/electrospray ionization-MS/MS method has been developed for the determination of dodine in fruit samples. Based on a liquid-liquid extraction of 10 g solid fruit homogenate using an acetone-dichloromethane-hexane mixture and acetate ammonium buffer (pH 4.5), this LC/MS/MS procedure was characterized by recoveries above 50%, with good intra-assay precision (RSD < 13%) and interassay precision (RSD < 18%) for seven different matrixes (apple, apricot, cherry, peach, pear, plum, and quince). This method was validated from 5 to 500 microg/kg according to standard guidelines. Its LOD (1 microg/kg) and LOQ (5 microg/kg) were in accordance with recommendations of the European legislation defined for infant food [maximum residue level (MRL) = 10 microg/kg]. The whole procedure was finally tested on 1022 fruit samples intended for commercialization, both infant food samples and samples not intended in particular for babies. In this study, dodine was detected in 27 samples; none exhibited a concentration higher than the MRL.
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Article dans une revue
Journal of AOAC INTERNATIONAL, AOAC International, 2011, 94 (1), pp.300-6
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http://www.hal.inserm.fr/inserm-00926392
Contributeur : Pierre Marquet <>
Soumis le : jeudi 9 janvier 2014 - 15:10:46
Dernière modification le : vendredi 16 février 2018 - 15:25:03

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  • HAL Id : inserm-00926392, version 1
  • PUBMED : 21391507

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Rozenn Le Grand, Franck Saint-Marcoux, Sylvain Dulaurent, François-Ludovic Sauvage, Christian Moesch, et al.. Determination of dodine in fruit samples by liquid chromatography electrospray ionization-tandem mass spectrometry.. Journal of AOAC INTERNATIONAL, AOAC International, 2011, 94 (1), pp.300-6. 〈inserm-00926392〉

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