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Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve.
Taïwe G. S., Bum E. N., Talla E., Dimo T., Dawe A., Sinniger V., Bonaz B., Boumendjel A., De Waard M.
Journal of Ethnopharmacology 151, 1 (2014) 445-51 - http://www.hal.inserm.fr/inserm-00908988
Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve.
Germain Sotoing Taïwe () 1, 2, Elisabeth Ngo Bum3, Emmanuel Talla4, Théophile Dimo5, Amadou Dawe6, Valérie Sinniger1, Bruno Bonaz1, Ahcène Boumendjel7, 8, Michel De Waard1, 8
1 :  GIN - U836 - Grenoble Institut des Neurosciences
INSERM : U836 – Université Joseph Fourier - Grenoble I – CHU Grenoble – CEA : DSV/IRTSV
UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9
2 :  Department of Zoology and Animal Physiology
University of Buea
Faculty of Science, P.O. Box 63 - Buea
3 :  Department of Biological Sciences
University of Ngaoundere
Faculty of Science - P.O. Box 454 Ngaoundéré
4 :  Department of Chemistry
University of Ngaoundere
Faculty of Science - P.O. Box 454 Ngaoundéré
5 :  Department of Animal Biology and Physiology
University of Yaounde
Faculty of Science, P.O. Box 812, Yaoundé
6 :  Department of Chemistry
Higher Teachers' Training College – University of Maroua
P.O. Box 55 Maroua
7 :  DPM - Département de pharmacochimie moléculaire
CNRS : UMR5063 – Université Joseph Fourier - Grenoble I
Batiment E André Rassat 470 rue de la chimie - BP 53 38041 GRENOBLE Cedex 9
8 :  Smartox Biotechnologies
Smartox Biotechnologies
Floralis, Biopolis, 5 Avenue du Grand Sablon, 38700 La Tronche
INSERM U836, équipe 8, Stress et interactions neurodigestives
INSERM U836, équipe 3, Canaux calciques, fonctions et pathologies
ETHNOPHARMACOLOGICAL RELEVANCE: The roots of Nauclea latifolia Smith (Rubiaceae) popularly known as "koumkouma" is used in traditional Cameroonian medicine as neuropathic pain remedy and for the treatment of headache, inflammatory pain and convulsion. This study was conducted to evaluate the antinociceptive effects of the alkaloid fraction isolated from Nauclea latifolia in neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve in rat. MATERIALS AND METHODS: Bioactive-guided fractionation of the root extracts of Nauclea latifolia using the Von Frey in a rat model of neuropathic pain (Benett model), afforded a potent anti-hyperalgesic fraction IV. Further fractionation of this fraction was performed by high-performance liquid chromatography (HPLC), yielded eight sub-fractions (F1-F8) which were tested for antinociceptive effects. The alkaloid fraction (F3) collected by HPLC, exhibited potent antinociceptive effects, and the anti-allodynic and anti-hyperalgesic effects of this fraction (8, 16, 40 and 80 mg/kg) were determined using the von Frey and acetone tests respectively in a rat model of neuropathic pain. Rota-rod performance and catalepsy tests were used for the assessment of motor coordination. RESULTS: The alkaloid fraction (80 mg/kg) administered intraperitoneally induced a completely decreased hyperalgesia 90 min post-dosing. In the acetone test, the Nauclea latifolia fraction at 80mg/kg showed its maximal anti-allodynic effects 120 min post-injection. The areas under the curve (AUC) of the anti-allodynic or anti-hyperalgesic effects produced by the alkaloid fraction at 80 mg/kg were significantly (p<0.001) greater than the AUC of effects produced by vehicle in CCI rats. The alkaloid fraction did not exhibit any significant effects on the spontaneous locomotor activity of the mice in rota-rod performance and no sign of catalepsy was observed. CONCLUSION: The analysis of the effects, expressed as the time course of AUC, supports the traditional use of Nauclea latifolia in neuropathic pain therapy. The pharmacological and chemical studies are continuing in order to characterize the mechanism(s) responsible for this anti-hyperalgesic and anti-allodynic action and also to identify the active substances present in the roots extracts of Nauclea latifolia.
Sciences du Vivant/Neurosciences

Articles dans des revues avec comité de lecture
Journal of Ethnopharmacology (J Ethnopharmacol)
Publisher Elsevier
ISSN 0378-8741 

Nauclea latifolia – neuropthic pain – anti-hyperalgesic – anti-allodynic – traditional medicine
Alkaloids – Analgesics – Animals – Cell Survival – Chronic Disease – Dose-Response Relationship – Drug – Female – Male – Mice – Morphine – Neuralgia – Plant Roots – Rats – Wistar – Rubiaceae – Sciatic Nerve
Agence Universitaire de la Francophonie (Bourse Internationale de Formation à la Recherche Doctorale, AUF 2009/2010, Ref.: 1021FR/277/BAC 2009/JGZ/AS)
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Taiwe-2013_N_latifolia_and_Neurophatic_pain_AA.pdf(436.3 KB)