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Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve.
Taïwe G. S., Bum E. N., Talla E., Dimo T., Dawe A., Sinniger V., Bonaz B., Boumendjel A., De Waard M.
Journal of Ethnopharmacology (2013) epub ahead of print - http://www.hal.inserm.fr/inserm-00908988
(24263011)
Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve.
Germain Sotoing Taïwe () 1, 2, Elisabeth Ngo Bum3, Emmanuel Talla4, Théophile Dimo5, Amadou Dawe6, Valérie Sinniger1, Bruno Bonaz1, Ahcène Boumendjel7, 8, Michel De Waard1, 8
1 :  GIN - U836 - Grenoble Institut des Neurosciences
http://neurosciences.ujf-grenoble.fr/
INSERM : U836 – Université Joseph Fourier - Grenoble I – CHU Grenoble – CEA : DSV/IRTSV
UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9
France
2 :  Department of Zoology and Animal Physiology
University of Buea
Faculty of Science, P.O. Box 63 - Buea
Cameroun
3 :  Department of Biological Sciences
University of Ngaoundere
Faculty of Science - P.O. Box 454 Ngaoundéré
Cameroun
4 :  Department of Chemistry
University of Ngaoundere
Faculty of Science - P.O. Box 454 Ngaoundéré
Cameroun
5 :  Department of Animal Biology and Physiology
University of Yaounde
Faculty of Science, P.O. Box 812, Yaoundé
Cameroun
6 :  Department of Chemistry
Higher Teachers' Training College – University of Maroua
P.O. Box 55 Maroua
Cameroun
7 :  DPM - Département de pharmacochimie moléculaire
http://www.ujf-grenoble.fr/ujf/fr/recherche/labujf/gpmfranc.phtml
CNRS : UMR5063 – Université Joseph Fourier - Grenoble I
Batiment E André Rassat 470 rue de la chimie - BP 53 38041 GRENOBLE Cedex 9
France
8 :  Smartox Biotechnologies
Smartox Biotechnologies
Floralis, Biopolis, 5 Avenue du Grand Sablon, 38700 La Tronche
France
INSERM U836, équipe 8, Stress et interactions neurodigestives
INSERM U836, équipe 3, Canaux calciques, fonctions et pathologies
: Ethnopharmacological relevance: The roots of Nauclea latifolia Smith (Rubiaceae) popularly known as "koumkouma" is used in traditional Cameroonian medicine as neuropathic pain remedy and for the treatment of headache, inflammatory pain and convulsion. AIM OF THE STUDY: This study was conducted to evaluate the antinociceptive effects of the alkaloid fraction isolated from Nauclea latifolia in neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve in rat. MATERIALS AND METHODS: Bioactive-guided fractionation of the root extracts of Nauclea latifolia using the Von Frey in a rat model of neuropathic pain (Benett model), afforded a potent anti-hyperalgesic fraction IV. Further fractionation of this fraction was performed by high-performance liquid chromatography (HPLC), yielded eight sub-fractions (F1-F8) which were tested for antinociceptive effects. The alkaloid fraction (F3) collected by HPLC, exhibited potent antinociceptive effects, and the anti-allodynic and anti-hyperalgesic effects of this fraction (8, 16, 40 and 80mg/kg) were determined using the von Frey and acetone tests respectively in a rat model of neuropathic pain. Rota-rod performance and catalepsy tests were used for the assessment of motor coordination. RESULTS: The alkaloid fraction (80mg/kg) administered intraperitoneally induced a completely decreased hyperalgesia 90min post-dosing. In the acetone test, the Nauclea latifolia fraction at 80mg/kg showed its maximal anti-allodynic effects 120min post-injection. The areas under the curve (AUC) of the anti-allodynic or anti-hyperalgesic effects produced by the alkaloid fraction at 80mg/kg were significantly (p<0.001) greater than the AUC of effects produced by vehicle in CCI rats. The alkaloid fraction did not exhibit any significant effects on the spontaneous locomotor activity of the mice in rota-rod performance and no sign of catalepsy was observed. CONCLUSION: The analysis of the effects, expressed as the time course of AUC, supports the traditional use of Nauclea latifolia in neuropathic pain therapy. The pharmacological and chemical studies are continuing in order to characterize the mechanism(s) responsible for this anti-hyperalgesic and anti-allodynic action and also to identify the active substances present in the roots extracts of Nauclea latifolia.
Sciences du Vivant/Neurosciences
Anglais
1872-7573

Articles dans des revues avec comité de lecture
10.1016/j.jep.2013.10.068
Journal of Ethnopharmacology (J Ethnopharmacol)
Publisher Elsevier
ISSN 0378-8741 
internationale
18/11/2013
18/11/2013
epub ahead of print

Nauclea latifolia – neuropthic pain – anti-hyperalgesic – anti-allodynic – traditional medicine
Agence Universitaire de la Francophonie (Bourse Internationale de Formation à la Recherche Doctorale, AUF 2009/2010, Ref.: 1021FR/277/BAC 2009/JGZ/AS)
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